Abstract
In vitro experiments, a crucial component of preclinical research, are widely used due to their accessibility and controlled conditions. However, traditional two-dimensional (2D) cell models are limited in their ability to simulate the complex interactions in organ systems. To address it, emerging technologies have shifted cell cultures from 2D to three-dimensional (3D), offering improved in vitro–in vivo correlation for traditional in vitro screening. Reconstructed human epidermis (RHE) is a 3D skin tissue model that closely mimics human skin in both structure and function. We established a sodium dodecyl sulfate (SDS)-induced epidermal injury model on RHE, and the result demonstrated that treating RHE with a 2.5 mg/mL SDS solution for 24 h could cause a significant epidermal damage. We also treated it with common clinical repair biomaterials, to screen the key indicator of SDS-induced 3D epidermal injury model, which includes several chemokines such as regulated upon activation normal T-cell expressed and secreted and interferon-γ-induced protein 10 that triggered inflammatory responses, and the important component protein of barrier structure—filaggrin and loricrin. In this study, we provide a platform for biomaterials evaluation that offers support and complementarities for in vitro experiments of skin repair.
Impact Statement
Two-dimensional (2D) cell cultures has been widely used to screen suitable conditions before in vivo experiments but owing to its limitations of functional and lack of complex interaction observed in vivo, more and more cell trials are moving from 2D to three-dimensional (3D). In this article, we established a sodium dodecyl sulfate-induced in vitro epidermal model in 3D cell cultures for the first time and identified appropriate indicators by treating it with several mature biomaterials for further evaluation of skin repair materials.
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