The GID SVF-1 and GID SVF-2 are closed-system, disposable, scalable cellular isolation devices designed for isolating the human adipose-derived stromal vascular fraction (hSVF) from lipoaspirates at the clinical point of care. The purpose of this study was to characterize the device performance with respect to cell yield and viability of the hSVF, as well as the hSVF purity and cellular composition. Our results demonstrate that adipose-derived hSVF can be safely obtained using both devices and standardized methods, yielding cells that are free of bacterial contaminants as measured by endotoxin levels, Gram stain, and culture. In addition, they produce cellular preparations with compositions consistent with reported values within the peer-reviewed literature using similar methods.
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References
1.
BourinP., et al.Stromal cells from the adipose tissue-derived stromal vascular fraction and culture expanded adipose tissue-derived stromal/stem cells: a joint statement of the International Federation for Adipose Therapeutics and Science (IFATS) and the International Society for Cellular Therapy (ISCT). Cytotherapy, 15, 641, 2013.
2.
DongZ., et al.The survival condition and immunoregulatory function of adipose stromal vascular fraction (SVF) in the early stage of nonvascularized adipose transplantation. PLoS One, 8, e80364, 2013.
3.
LinS.D., et al.Engineering adipose tissue from uncultured human adipose stromal vascular fraction on gelatin sponge and collagen matrix scaffolds. Tissue Eng Part A, 17, 1489, 2011.
4.
RiordanN.H., et al.Non-expanded adipose stromal vascular fraction cell therapy for multiple sclerosis. J Transl Med, 7, 29, 2009.
5.
RodbellM.Localization of lipoprotein lipase in fat cells of rat adipose tissue. J Biol Chem, 239, 753, 1964.
6.
RodbellM.Metabolism of isolated fat cells. I. Effects of hormones on glucose metabolism and lipolysis. J Biol Chem, 239, 375, 1964.
7.
ZukP.A., et al.Multilineage cells from human adipose tissue: implications for cell-based therapies. Tissue Eng, 7, 211, 2001.
8.
LeviB., et al.Dura mater stimulates human adipose-derived stromal cells to undergo bone formation in mouse calvarial defects. Stem Cells, 29, 1241, 2011.
9.
EricksonG.R., et al.Chondrogenic potential of adipose tissue-derived stromal cells in vitro and in vivo. Biochem Biophys Res Commun, 290, 763, 2002.
10.
SeoM.J., et al.Differentiation of human adipose stromal cells into hepatic lineage in vitro and in vivo. Biochem Biophys Res Commun, 328, 258, 2005.
11.
TraktuevD.O., et al.Robust functional vascular network formation in vivo by cooperation of adipose progenitor and endothelial cells. Circ Res, 104, 1410, 2009.
12.
AronowitzJ.A., and EllenhornJ.D.Adipose stromal vascular fraction isolation: a head-to-head comparison of four commercial cell separation systems. Plast Reconstr Surg, 132, 932e, 2013.
13.
Dos AnjosS., et al.Reproducible volume restoration and efficient long-term volume retention after point-of-care standardized cell-enhanced fat grafting in breast surgery. Plast Reconstr Surg Glob Open, 3, e547, 2015.
14.
FodorP.B., and PaulsethS.G.Adipose derived stromal cell (ADSC) injections for pain management of osteoarthritis in the human knee joint. Aesthet Surg J, 36, 229, 2016.
15.
GarzaJ., et al.Use of autologous adipose-derived stromal vascular fraction to treat osteoarthritis of the knee: a feasibility and safety study. J Regen Med, 03, 2014.
16.
ZimmerlinL., et al.Stromal vascular progenitors in adult human adipose tissue. Cytometry A, 77, 22, 2010.
17.
FaustiniM., et al.Nonexpanded mesenchymal stem cells for regenerative medicine: yield in stromal vascular fraction from adipose tissues. Tissue Eng Part C Methods, 16, 1515, 2010.
18.
FraserJ.K., et al.Differences in stem and progenitor cell yield in different subcutaneous adipose tissue depots. Cytotherapy, 9, 459, 2007.
19.
Oedayrajsingh-VarmaM.J., et al.Adipose tissue-derived mesenchymal stem cell yield and growth characteristics are affected by the tissue-harvesting procedure. Cytotherapy, 8, 166, 2006.
20.
Dos-Anjos VilaboaS., Navarro-PalouM., and LlullR.Age influence on stromal vascular fraction cell yield obtained from human lipoaspirates. Cytotherapy, 16, 1092, 2014.
21.
SicaG.Bias in research studies. Radiology, 238, 780, 2006.
22.
LinK., et al.Characterization of adipose tissue-derived cells isolated with the Celution system. Cytotherapy, 10, 417, 2008.
23.
DoiK., et al.Stromal vascular fraction isolated from lipo-aspirates using an automated processing system: bench and bed analysis. J Tissue Eng Regen Med, 7, 864, 2013.
24.
FraserJ., et al.The Celution System: automated processing of adipose-derived regenerative cells in a functionally closed system. Adv Wound Care, 3, 38, 2013.
25.
AstoriG., et al.“In vitro” and multicolor phenotypic characterization of cell subpopulations identified in fresh human adipose tissue stromal vascular fraction and in the derived mesenchymal stem cells. J Transl Med, 5, 55, 2007.
26.
ChoudheryM.S., et al.Subcutaneous adipose tissue-derived stem cell utility is independent of anatomical harvest site. Biores Open Access, 4, 131, 2015.
27.
PilgaardL., et al.Comparative analysis of highly defined proteases for the isolation of adipose tissue-derived stem cells. Regen Med, 3, 705, 2008.
28.
MitchellJ.B., et al.Immunophenotype of human adipose-derived cells: temporal changes in stromal-associated and stem cell-associated markers. Stem Cells, 24, 376, 2006.
29.
ZimmerlinL., et al.Human adipose stromal vascular cell delivery in a fibrin spray. Cytotherapy, 15, 102, 2013.
30.
YoshimuraK., et al.Characterization of freshly isolated and cultured cells derived from the fatty and fluid portions of liposuction aspirates. J Cell Physiol, 208, 64, 2006.
31.
WynnT., and BarronL.Macrophages: master regulators of inflammation and fibrosis. Semin Liver Dis, 30, 245, 2010.
32.
GordonS., and MartinezF.O.Alternative activation of macrophages: mechanism and functions. Immunity, 32, 593, 2010.
33.
GordonS., and TaylorP.R.Monocyte and macrophage heterogeneity. Nat Rev Immunol, 5, 953, 2005.
34.
MosserD.M.The many faces of macrophage activation. J Leukoc Biol, 73, 209, 2003.
35.
StoutR.D., et al.Macrophages sequentially change their functional phenotype in response to changes in microenvironmental influences. J Immunol, 175, 342, 2005.
36.
MillsC.D.M1 and M2 macrophages: oracles of health and disease. Crit Rev Immunol, 32, 463, 2012.
37.
ZhaoY., GlesneD., and HubermanE.A human peripheral blood monocyte-derived subset acts as pluripotent stem cells. Proc Natl Acad Sci U S A, 100, 2426, 2003.
38.
PufeT., et al.Programmable cells of monocytic origin (PCMO): a source of peripheral blood stem cells that generate collagen type II-producing chondrocytes. J Orthop Res, 26, 304, 2008.
39.
UngefrorenH., and FandrichF.The programmable cell of monocytic origin (PCMO): a potential adult stem/progenitor cell source for the generation of islet cells. Adv Exp Med Biol, 654, 667, 2010.
ChangE.I., et al.Age decreases endothelial progenitor cell recruitment through decreases in hypoxia-inducible factor 1alpha stabilization during ischemia. Circulation, 116, 2818, 2007.
42.
SidneyL.E., et al.Concise review: evidence for CD34 as a common marker for diverse progenitors. Stem Cells, 32, 1380, 2014.
43.
GronthosS., et al.Surface protein characterization of human adipose tissue-derived stromal cells. J Cell Physiol, 189, 54, 2001.
44.
ZannettinoA.C., et al.Multipotential human adipose-derived stromal stem cells exhibit a perivascular phenotype in vitro and in vivo. J Cell Physiol, 214, 413, 2008.
45.
ToyodaM., et al.Characterization and comparison of adipose tissue-derived cells from human subcutaneous and omental adipose tissues. Cell Biochem Funct, 27, 440, 2009.
46.
ElshalM.F., et al.CD146 (Mel-CAM), an adhesion marker of endothelial cells, is a novel marker of lymphocyte subset activation in normal peripheral blood. Blood, 106, 2923, 2005.
47.
AmosP.J., et al.IFATS collection: the role of human adipose-derived stromal cells in inflammatory microvascular remodeling and evidence of a perivascular phenotype. Stem Cells, 26, 2682, 2008.
48.
CovasD.T., et al.Multipotent mesenchymal stromal cells obtained from diverse human tissues share functional properties and gene-expression profile with CD146+ perivascular cells and fibroblasts. Exp Hematol, 36, 642, 2008.
49.
CrisanM., et al.A perivascular origin for mesenchymal stem cells in multiple human organs. Cell Stem Cell, 3, 301, 2008.
50.
TraktuevD.O., et al.A population of multipotent CD34-positive adipose stromal cells share pericyte and mesenchymal surface markers, reside in a periendothelial location, and stabilize endothelial networks. Circ Res, 102, 77, 2008.
51.
RussellK.C., et al.In vitro high-capacity assay to quantify the clonal heterogeneity in trilineage potential of mesenchymal stem cells reveals a complex hierarchy of lineage commitment. Stem Cells, 28, 788, 2010.
GuilakF., et al.Clonal analysis of the differentiation potential of human adipose-derived adult stem cells. J Cell Physiol, 206, 229, 2006.
54.
KernS., et al.Comparative analysis of mesenchymal stem cells from bone marrow, umbilical cord blood, or adipose tissue. Stem Cells, 24, 1294–1301, 2006.
55.
BadalamenteM.A., HurstL.C., and HentzV.R.Collagen as a clinical target: nonoperative treatment of Dupuytren's disease. J Hand Surg Am, 27, 788, 2002.
56.
ThomasA., and BayatA.The emerging role of Clostridium histolyticum collagenase in the treatment of Dupuytren disease. Ther Clin Risk Manag, 6, 557, 2010.
57.
DomenisR., et al.Adipose tissue derived stem cells: in vitro and in vivo analysis of a standard and three commercially available cell-assisted lipotransfer techniques. Stem Cell Res Therapy, 6, 2, 2015.
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