Fabrication of Hydroxypropyl Chitosan/Soy Protein Isolate Hydrogel for Effective Hemorrhage Control

Abstract

Hemostatic materials are increasingly important in civilian and military clinics. In this work, a hydrogel was fabricated from hydroxypropyl chitosan (HPCS) and soy protein isolate (SPI) through the crosslinking of epichlorohydrin. Effects of SPI content on the structure, and physical and biological properties of the prepared hydrogels were characterized using Fourier-transform infrared spectroscopy, X-ray diffractometry, scanning electron microscopy, water uptake testing, mechanical properties testing, MTT assay, hemolysis ratio testing, and routine blood coagulation test. The results indicated that the hydrogels showed high water uptake ability and compressive strength. The in vitro biocompatibility evaluation revealed that the hydrogel contains 30% SPI content (HCSH-30), could promote blood coagulation and cell proliferation. Furthermore, the hemostatic model of liver in New Zealand rabbit was applied to assess the hemostatic efficacy of the hydrogels. The results demonstrated that HCSH-30 stopped bleeding in 75 ± 1.63 s and improved hemostasis as compared with medical gauze. Thus, the HPCS/SPI hydrogel is expected to be a potential candidate for effective hemorrhage control.

Impact statement

Stoppage of bleeding is of paramount clinical significance in prophylactic, surgical, and emergency scenarios. This work describes a hydroxypropyl chitosan (HPCS)/soy protein isolate hydrogel, which could promote blood coagulation and cell proliferation, as well as stop bleeding in 75 ± 1.63 s on the liver of New Zealand rabbits. Thus, we provide a new candidate for hemostatic material and broaden the application of HPCS-based materials.