Live-Cell,Temporal Gene Expression Analysis of Osteogenic Differentiation in Adipose-Derived Stem Cells

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References

Donizetti-Oliveira

, Semedo

, Burgos-Silva

, Cenedeze

M.A.

, Malheiros

D.M.

, Reis

M.A.

et al. Adipose tissue-derived stem cell treatment prevents renal disease progression. Cell Transplant, 2012Epub ahead of print10.3727/096368911x623-925.

Gimble

J.M.

, Grayson

, Guilak

, Lopez

M.J.

, Vunjak-Novakovic

Adipose tissue as a stem cell source for musculoskeletal regeneration. Front Biosci (Schol Ed), 3:69. 2011.

Guilak

, Lott

K.E.

, Awad

H.A.

, Cao

, Hicok

K.C.

, Fermor

et al. Clonal analysis of the differentiation potential of human adipose-derived adult stem cells. J Cell Physiol, 206:229. 2006.

Guilak

, Estes

B.T.

, Diekman

B.O.

, Moutos

F.T.

, Gimble

J.M.

2010 Nicolas Andry Award: Multipotent adult stem cells from adipose tissue for musculoskeletal tissue engineering. Clin Orthop Relat Res, 468:2530. 2010.

Locke

, Feisst

, Dunbar

P.R.

Concise review: human adipose-derived stem cells: separating promise from clinical need. Stem Cells, 29:404. 2011.

Yoshimura

, Shigeura

, Matsumoto

, Sato

, Takaki

, Aiba-Kojima

et al. Characterization of freshly isolated and cultured cells derived from the fatty and fluid portions of liposuction aspirates. J Cell Physiol, 208:64. 2006.

Gimble

J.M.

, Guilak

, Nuttall

M.E.

, Sathishkumar

, Vidal

, Bunnell

B.A.

In vitro differentiation potential of mesenchymal stem cells. Transfus Med Hemother, 35:228. 2008.

Zuk

P.A.

, Zhu

, Mizuno

, Huang

, Futrell

J.W.

, Katz

A.J.

et al. Multilineage cells from human adipose tissue: implications for cell-based therapies. Tissue Eng, 7:211. 2001.

Russell

K.C.

, Phinney

D.G.

, Lacey

M.R.

, Barrilleaux

B.L.

, Meyertholen

K.E.

, O'Connor

K.C.

In vitro high-capacity assay to quantify the clonal heterogeneity in trilineage potential of mesenchymal stem cells reveals a complex hierarchy of lineage commitment. Stem Cells, 28:788. 2010.

10.

Tyagi

, Kramer

F.R.

Molecular beacons: probes that fluoresce upon hybridization. Nat Biotechnol, 14:303. 1996.

11.

Manganelli

, Tyagi

, Smith

Real time PCR using molecular beacons: a new tool to identify point mutations and to analyze gene expression in mycobacterium tuberculosis. Methods Mol Med, 54:295. 2001.

12.

Mhlanga

M.M.

, Vargas

D.Y.

, Fung

C.W.

, Kramer

F.R.

, Tyagi

tRNA-linked molecular beacons for imaging mRNAs in the cytoplasm of living cells. Nucleic Acids Res, 33:1902. 2005.

13.

Rhee

W.J.

, Bao

Simultaneous detection of mRNA and protein stem cell markers in live cells. BMC Biotechnol, 9:30. 2009.

14.

Santangelo

P.J.

, Nix

, Tsourkas

, Bao

Dual FRET molecular beacons for mRNA detection in living cells. Nucleic Acids Res, 32:e57. 2004.

15.

Tsourkas

, Bao

Shedding light on health and disease using molecular beacons. Brief Funct Genomic Proteomic, 1:372. 2003.

16.

Baker

M.B.

, Bao

, Searles

C.D.

In vitro quantification of specific microRNA using molecular beacons. Nucleic Acids Res, 40:e13. 2012.

17.

Kim

E.K.

, Lim

, Park

J.M.

, Seo

J.K.

, Kim

J.H.

, Kim

K.T.

et al. Human mesenchymal stem cell differentiation to the osteogenic or adipogenic lineage is regulated by AMP-activated protein kinase. J Cell Physiol, 227:1680. 2012.

18.

Abrahamsson

C.K.

, Yang

, Park

, Brunger

J.M.

, Valonen

P.K.

, Langer

et al. Chondrogenesis and mineralization during in vitro culture of human mesenchymal stem cells on three-dimensional woven scaffolds. Tissue Eng Part A, 16:3709. 2010.

19.

Estes

B.T.

, Diekman

B.O.

, Guilak

Monolayer cell expansion conditions affect the chondrogenic potential of adipose-derived stem cells. Biotechnol Bioeng, 99:986. 2008.

20.

Mathews

D.H.

, Turner

D.H.

, Zuker

RNA secondary structure prediction. Beaucage

S.L.

et al. Current Protocols in Nucleic Acid Chemistry. Hoboken, NJ: John Wiley & SonsChapter 11, Unit 11.2.2007.

21.

Zuker

Mfold web server for nucleic acid folding and hybridization prediction. Nucleic Acids Res, 31:3406. 2003.

22.

Mount

D.W.

Using the Basic Local Alignment Search Tool (BLAST)

CSH Protoc, 2007pdb top172007.

23.

Altschul

S.F.

, Gish

, Miller

, Myers

E.W.

, Lipman

D.J.

Basic local alignment search tool. J Mol Biol, 215:403. 1990.

24.

Bao

, Rhee

W.J.

, Tsourkas

Fluorescent probes for live-cell RNA detection. Annu Rev Biomed Eng, 11:25. 2009.

25.

Bratu

D.P.

, Catrina

I.E.

, Marras

S.A.

Tiny molecular beacons for in vivo mRNA detection. Methods Mol Biol, 714:141. 2011.

26.

Rothem

D.E.

, Rothem

, Dahan

, Eliakim

, Soudry

Nicotinic modulation of gene expression in osteoblast cells, MG-63. Bone, 48:903. 2011.

27.

Mueller

S.M.

, Glowacki

Age-related decline in the osteogenic potential of human bone marrow cells cultured in three-dimensional collagen sponges. J Cell Biochem, 82:583. 2001.

28.

Santangelo

P.J.

, Nitin

, Bao

Direct visualization of mRNA colocalization with mitochondria in living cells using molecular beacons. J Biomed Opt, 10:44025. 2005.

29.

Bunnell

B.A.

, Estes

B.T.

, Guilak

, Gimble

J.M.

Differentiation of adipose stem cells. Methods Mol Biol, 456:155. 2008.

30.

Lamprecht

M.R.

, Sabatini

D.M.

, Carpenter

A.E.

CellProfiler: free, versatile software for automated biological image analysis. BioTechniques, 42:71. 2007.

31.

Carpenter

A.E.

, Jones

T.R.

, Lamprecht

M.R.

, Clarke

, Kang

I.H.

, Friman

et al. CellProfiler: image analysis software for identifying, quantifying cell phenotypes. Genome Biol, 7:R100. 2006.

32.

Vet

J.A.

, Marras

S.A.

Design and optimization of molecular beacon real-time polymerase chain reaction assays. Methods Mol Biol, 288:273. 2005.

33.

Tsai

K.S.

, Kao

S.Y.

, Wang

C.Y.

, Wang

Y.J.

, Wang

J.P.

, Hung

S.C.

Type I collagen promotes proliferation and osteogenesis of human mesenchymal stem cells via activation of ERK and Akt pathways. J Biomed Mater Res Part A, 94:673. 2010.

34.

Frank

, Heim

, Jakob

, Barbero

, Schafer

, Bendik

et al. Real-time quantitative RT-PCR analysis of human bone marrow stromal cells during osteogenic differentiation in vitro. J Cell Biochem, 85:737. 2002.

35.

Pittenger

M.F.

, Mackay

A.M.

, Beck

S.C.

, Jaiswal

R.K.

, Douglas

, Mosca

J.D.

et al. Multilineage potential of adult human mesenchymal stem cells. Science, 284:143. 1999.

36.

Zuk

P.A.

, Zhu

, Ashjian

, De Ugarte

D.A.

, Huang

J.I.

, Mizuno

et al. Human adipose tissue is a source of multipotent stem cells. Mol Biol Cell, 13:4279. 2002.

37.

King

F.W.

, Liszewski

, Ritner

, Bernstein

H.S.

High-throughput tracking of pluripotent human embryonic stem cells with dual fluorescence resonance energy transfer molecular beacons. Stem Cells Dev, 20:475. 2011.

38.

Estes

B.T.

, Wu

A.W.

, Storms

R.W.

, Guilak

Extended passaging, but not aldehyde dehydrogenase activity, increases the chondrogenic potential of human adipose-derived adult stem cells. J Cell Physiol, 209:987. 2006.

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