The transcription factor Pax7 plays a key role during embryonic myogenesis and sustains the proper function of satellite cells, which serve as adult skeletal muscle stem cells. Overexpression of Pax7 has been shown to promote the myogenic differentiation of pluripotent stem cells. However, the effects of the absence of functional Pax7 in differentiating embryonic stem cells (ESCs) have not yet been directly tested. Herein, we studied mouse stem cells that lacked a functional Pax7 gene and characterized the differentiation of these stem cells under conditions that promoted the derivation of myoblasts in vitro. We analyzed the expression of myogenic factors, such as myogenic regulatory factors and muscle-specific microRNAs, in wild-type and mutant cells. Finally, we compared the transcriptome of both types of cells and did not find substantial differences in the expression of genes related to the regulation of myogenesis. As a result, we showed that the absence of functional Pax7 does not prevent the in vitro myogenic differentiation of ESCs.
MossFP and LeblondCP. (1971). Satellite cells as the source of nuclei in muscles of growing rats. Anat Rec, 170:421–435.
3.
KatzB. (1961). The terminations of the afferent nerve fibre in the muscle spindle of the frog. Philos Trans R Soc Lond B Biol Sci, 243:221–240.
4.
Yablonka-ReuveniZ. (2011). The skeletal muscle satellite cell: still young and fascinating at 50. J Histochem Cytochem, 59:1041–1059.
5.
ScharnerJ and ZammitPS. (2011). The muscle satellite cell at 50: the formative years. Skelet Muscle, 1:28.
6.
RelaixF, MontarrasD, ZaffranS, Gayraud-MorelB, RocancourtD, TajbakhshS, MansouriA, CumanoA and BuckinghamM. (2006). Pax3 and Pax7 have distinct and overlapping functions in adult muscle progenitor cells. J Cell Biol, 172:91–102.
7.
BoberE, FranzT, ArnoldHH, GrussP and TremblayP. (1994). Pax-3 is required for the development of limb muscles: a possible role for the migration of dermomyotomal muscle progenitor cells. Development, 120:603–612.
8.
WilliamsBA and OrdahlCP. (1994). Pax-3 expression in segmental mesoderm marks early stages in myogenic cell specification. Development, 120:785–796.
9.
BraunT, BoberE, RudnickiMA, JaenischR and ArnoldHH. (1994). MyoD expression marks the onset of skeletal myogenesis in Myf-5 mutant mice. Development, 120:3083–3092.
10.
TajbakhshS, RocancourtD, CossuG and BuckinghamM. (1997). Redefining the genetic hierarchies controlling skeletal myogenesis: Pax-3 and Myf-5 act upstream of MyoD. Cell, 89:127–138.
11.
OlguinHC, YangZ, TapscottSJ and OlwinBB. (2007). Reciprocal inhibition between Pax7 and muscle regulatory factors modulates myogenic cell fate determination. J Cell Biol, 177:769–779.
12.
RelaixF, RocancourtD, MansouriA and BuckinghamM. (2005). A Pax3/Pax7-dependent population of skeletal muscle progenitor cells. Nature, 435:948–953.
13.
SassoonD, LyonsG, WrightWE, LinV, LassarA, WeintraubH and BuckinghamM. (1989). Expression of two myogenic regulatory factors myogenin and MyoD1 during mouse embryogenesis. Nature, 341:303–307.
14.
SealeP, SabourinLA, Girgis-GabardoA, MansouriA, GrussP and RudnickiMA. (2000). Pax7 is required for the specification of myogenic satellite cells. Cell, 102:777–786.
15.
FeldmanJL and StockdaleFE. (1992). Temporal appearance of satellite cells during myogenesis. Dev Biol, 153:217–226.
16.
HorstD, SergiC, MikuzG, JuergensH and VorobyovE. (2005). Comparative expression analysis of Pax3 and Pax7 during myogenesis in the mouse embryo. J Pathol, 207:3–3.
17.
GrabowskaI, ArchackaK, CzerwinskaAM, KrupaM and CiemerychMA. (2012). Mouse and human pluripotent stem cells and the means of their myogenic differentiation. Results Probl Cell Differ, 55:321–356.
18.
RelaixF, RocancourtD, MansouriA and BuckinghamM. (2004). Divergent functions of murine Pax3 and Pax7 in limb muscle development. Genes Dev, 18:1088–1105.
19.
KumarD, ShadrachJL, WagersAJ and LassarAB. (2009). Id3 is a direct transcriptional target of Pax7 in quiescent satellite cells. Mol Biol Cell, 20:3170–3177.
20.
OlguinHC and OlwinBB. (2004). Pax-7 up-regulation inhibits myogenesis and cell cycle progression in satellite cells: a potential mechanism for self-renewal. Dev Biol, 275:375–388.
21.
McKinnellIW, IshibashiJ, Le GrandF, PunchVG, AddicksGC, GreenblattJF, DilworthFJ and RudnickiMA. (2008). Pax7 activates myogenic genes by recruitment of a histone methyltransferase complex. Nat Cell Biol, 10:77–84.
22.
CristCG, MontarrasD and BuckinghamM. (2012). Muscle satellite cells are primed for myogenesis but maintain quiescence with sequestration of Myf5 mRNA targeted by microRNA-31 in mRNP granules. Cell Stem Cell, 11:118–126.
23.
CristCG, MontarrasD, PallafacchinaG, RocancourtD, CumanoA, ConwaySJ and BuckinghamM. (2009). Muscle stem cell behavior is modified by microRNA-27 regulation of Pax3 expression. Proc Natl Acad Sci U S A, 106:13383–13387.
24.
ChenJF, TaoYZ, LiJA, DengZL, YanZ, XiaoXA and WangDZ. (2010). microRNA-1 and microRNA-206 regulate skeletal muscle satellite cell proliferation and differentiation by repressing Pax7. J Cell Biol, 190:867–879.
25.
DeyBK, GaganJ and DuttaA. (2011). miR-206 and -486 induce myoblast differentiation by downregulating Pax7. Mol Cell Biol, 31:203–214.
26.
RaoPK, KumarRM, FarkhondehM, BaskervilleS and LodishHF. (2006). Myogenic factors that regulate expression of muscle-specific microRNAs. Proc Natl Acad Sci U S A, 103:8721–8726.
27.
LiuN, WilliamsAH, KimY, McAnallyJ, BezprozvannayaS, SutherlandLB, RichardsonJA, Bassel-DubyR and OlsonEN. (2007). An intragenic MEF2-dependent enhancer directs muscle-specific expression of microRNAs 1 and 133. Proc Natl Acad Sci U S A, 104:20844–20849.
28.
SweetmanD, GoljanekK, RathjenT, OustaninaS, BraunT, DalmayT and MunsterbergA. (2008). Specific requirements of MRFs for the expression of muscle specific microRNAs, miR-1, miR-206 and miR-133. Dev Biol, 321:491–499.
29.
MansouriA, StoykovaA, TorresM and GrussP. (1996). Dysgenesis of cephalic neural crest derivatives in Pax7-/- mutant mice. Development, 122:831–838.
30.
SealeP, PolesskayaA and RudnickiMA. (2003). Adult stem cell specification by Wnt signaling in muscle regeneration. Cell Cycle, 2:418–419.
31.
OustaninaS, HauseG and BraunT. (2004). Pax7 directs postnatal renewal and propagation of myogenic satellite cells but not their specification. EMBO J, 23:3430–3439.
32.
KuangS, ChargeSB, SealeP, HuhM and RudnickiMA. (2006). Distinct roles for Pax7 and Pax3 in adult regenerative myogenesis. J Cell Biol, 172:103–113.
33.
HutchesonDA, ZhaoJ, MerrellA, HaldarM and KardonG. (2009). Embryonic and fetal limb myogenic cells are derived from developmentally distinct progenitors and have different requirements for beta-catenin. Genes Dev, 23:997–1013.
34.
WorlJ, BreuerC and NeuhuberWL. (2009). Deletion of Pax7 changes the tunica muscularis of the mouse esophagus from an entirely striated into a mixed phenotype. Dev Dyn, 238:864–874.
35.
LepperC, ConwaySJ and FanCM. (2009). Adult satellite cells and embryonic muscle progenitors have distinct genetic requirements. Nature, 460:627–631.
36.
GuntherS, KimJ, KostinS, LepperC, FanCM and BraunT. (2013). Myf5-positive satellite cells contribute to Pax7-dependent long-term maintenance of adult muscle stem cells. Cell Stem Cell, 13:590–601.
37.
von MaltzahnJ, JonesAE, ParksRJ and RudnickiMA. (2013). Pax7 is critical for the normal function of satellite cells in adult skeletal muscle. Proc Natl Acad Sci U S A, 110:16474–16479.
38.
WangJ and ConboyI. (2009). Embryonic vs. adult myogenesis: challenging the 'regeneration recapitulates development' paradigm. J Mol Cell Biol, 2:1–4.
39.
RelaixF and ZammitPS. (2012). Satellite cells are essential for skeletal muscle regeneration: the cell on the edge returns centre stage. Development, 139:2845–2856.
40.
SambasivanR, YaoR, KissenpfennigA, Van WittenbergheL, PaldiA, Gayraud-MorelB, GuenouH, MalissenB, TajbakhshS and GalyA. (2011). Pax7-expressing satellite cells are indispensable for adult skeletal muscle regeneration. Development, 138:3647–3656.
MurphyMM, LawsonJA, MathewSJ, HutchesonDA and KardonG. (2011). Satellite cells, connective tissue fibroblasts and their interactions are crucial for muscle regeneration. Development, 138:3625–3637.
43.
LepperC, PartridgeTA and FanCM. (2011). An absolute requirement for Pax7-positive satellite cells in acute injury-induced skeletal muscle regeneration. Development, 138:3639–3646.
44.
EckardtS, McLaughlinKJ and WillenbringH. (2011). Mouse chimeras as a system to investigate development, cell and tissue function, disease mechanisms and organ regeneration. Cell Cycle, 10:2091–2099.
45.
EgganK, RodeA, JentschI, SamuelC, HennekT, TintrupH, ZevnikB, ErwinJ, LoringJ, et al. (2002). Male and female mice derived from the same embryonic stem cell clone by tetraploid embryo complementation. Nat Biotechnol, 20:455–459.
46.
WangZ and JaenischR. (2004). At most three ES cells contribute to the somatic lineages of chimeric mice and of mice produced by ES-tetraploid complementation. Dev Biol, 275:192–201.
47.
ZhouD, RenJX, RyanTM, HigginsNP and TownesTM. (2004). Rapid tagging of endogenous mouse genes by recombineering and ES cell complementation of tetraploid blastocysts. Nucleic Acids Res, 32:e128.
48.
GroppM, ShiloV, VainerG, GovM, GilY, KhanerH, MatzrafiL, IdelsonM, KopolovicJ, ZakNB and ReubinoffBE. (2012). Standardization of the teratoma assay for analysis of pluripotency of human ES cells and biosafety of their differentiated progeny. PLoS One, 7:e45532.
49.
BorchinB, ChenJ and BarberiT. (2013). Derivation and FACS-Mediated Purification of PAX3+/PAX7+ Skeletal muscle precursors from human pluripotent stem cells. Stem Cell Rep, 1:620–631.
50.
SheltonM, MetzJ, LiuJ, CarpenedoRL, DemersS-P, StanfordWL and SkerjancIS. (2014). Derivation and expansion of PAX7-positive muscle progenitors from human and mouse embryonic stem cells. Stem Cell Rep, 3:516–529.
51.
ChalJ, OginumaM, Al TanouryZ, GobertB, SumaraO, HickA, BoussonF, ZidouniY, MurschC, et al. (2015). Differentiation of pluripotent stem cells to muscle fiber to model Duchenne muscular dystrophy. Nat Biotechnol, 33:962–969.
52.
CloseB, BanisterK, BaumansV, BernothEM, BromageN, BunyanJ, ErhardtW, FlecknellP, GregoryN, et al. (1996). Recommendations for euthanasia of experimental animals: Part 1. DGXI of the European Commission. Lab Anim, 30:293–316.
53.
CloseB, BanisterK, BaumansV, BernothEM, BromageN, BunyanJ, ErhardtW, FlecknellP, GregoryN, et al. (1997). Recommendations for euthanasia of experimental animals: Part 2. DGXT of the European Commission. Lab Anim, 31:1–32.
54.
RobertsonE. (1987). Embryo-derived stem cell lines. In: Teratocarcinomas and Embryonic Stem Cells: A Practical Approach. RobertsonE ed. Oxford University Press, Oxford, England, pp 71–112.
55.
FultonBP and WhittinghamDG. (1978). Activation of mammalian oocytes by intracellular injection of calcium. Nature, 273:149–151.
56.
BarberiT, BradburyM, DincerZ, PanagiotakosG, SocciND and StuderL. (2007). Derivation of engraftable skeletal myoblasts from human embryonic stem cells. Nat Med, 13:642–648.
57.
StavropoulosME, MengarelliI and BarberiT. (2009). Differentiation of multipotent mesenchymal precursors and skeletal myoblasts from human embryonic stem cells. Curr Protoc Stem Cell Biol, Chapter 1:Unit 1F:8.
58.
KennedyKA, PorterT, MehtaV, RyanSD, PriceF, PeshdaryV, KaramboulasC, SavageJ, DrysdaleTA, et al. (2009). Retinoic acid enhances skeletal muscle progenitor formation and bypasses inhibition by bone morphogenetic protein 4 but not dominant negative beta-catenin. BMC Biol, 7:67.
59.
WobusAM, GuanK, YangHT and BohelerKR. (2002). Embryonic stem cells as a model to study cardiac, skeletal muscle, and vascular smooth muscle cell differentiation. Methods Mol Biol, 185:127–156.
60.
CiemerychMA, YuQ, SzczepanskaK and SicinskiP. (2008). CDK4 activity in mouse embryos expressing a single D-type cyclin. Int J Dev Biol, 52:299–305.
61.
TamakiT, AkatsukaA, AndoK, NakamuraY, MatsuzawaH, HottaT, RoyRR and EdgertonVR. (2002). Identification of myogenic-endothelial progenitor cells in the interstitial spaces of skeletal muscle. J Cell Biol, 157:571–577.
62.
HoganBF, ConstantiniF and LacyE. (1986). Manipulating the Mouse Embryo: A Laboratory Manual. Cold Spring Harbor Laboratory, Cold Spring Harbor, NY.
63.
LivakKJ and SchmittgenTD. (2001). Analysis of relative gene expression data using real-time quantitative PCR and the 2(T)(-Delta Delta C) method. Methods, 25:402–408.
64.
BolstadBM, IrizarryRA, AstrandM and SpeedTP. (2003). A comparison of normalization methods for high density oligonucleotide array data based on variance and bias. Bioinformatics, 19:185–193.
65.
KawaseE, SuemoriH, TakahashiN, OkazakiK, HashimotoK and NakatsujiN. (1994). Strain Difference in Establishment of Mouse Embryonic Stem (Es) Cell-Lines. Int J Dev Biol, 38:385–390.
66.
DoetschmanTC, EistetterH, KatzM, SchmidtW and KemlerR. (1985). The in vitro development of blastocyst-derived embryonic stem cell lines: formation of visceral yolk sac, blood islands and myocardium. J Embryol Exp Morphol, 87:27–45.
67.
PeaseS and WilliamsRL. (1990). Formation of germ-line chimeras from embryonic stem cells maintained with recombinant leukemia inhibitory factor. Exp Cell Res, 190:209–211.
68.
SalaniS, DonadoniC, RizzoF, BresolinN, ComiGP and CortiS. (2012). Generation of skeletal muscle cells from embryonic and induced pluripotent stem cells as an in vitro model and for therapy of muscular dystrophies. J Cell Mol Med, 16:1353–1364.
69.
RohwedelJ, GuanK and WobusAM. (1999). Induction of cellular differentiation by retinoic acid in vitro. Cells Tissues Organs, 165:190–202.
70.
KataokaH, TakakuraN, NishikawaS, TsuchidaK, KodamaH, KunisadaT, RisauW, KitaT and NishikawaSI. (1997). Expressions of PDGF receptor alpha, c-Kit and Flk1 genes clustering in mouse chromosome 5 define distinct subsets of nascent mesodermal cells. Dev Growth Differ, 39:729–740.
71.
MooreJW, DionneC, JayeM and SwainJL. (1991). The Messenger-Rnas Encoding Acidic Fgf, Basic Fgf and Fgf receptor are coordinately down-regulated during myogenic differentiation. Development, 111:741–748.
72.
PevnyLH, SockanathanS, PlaczekM and Lovell-BadgeR. (1998). A role for SOX1 in neural determination. Development, 125:1967–1978.
73.
MorriseyEE, TangZ, SigristK, LuMM, JiangF, IpHS and ParmacekMS. (1998). GATA6 regulates HNF4 and is required for differentiation of visceral endoderm in the mouse embryo. Genes Dev, 12:3579–3590.
74.
CohenhaguenauerO, BartonPJR, NguyenVC, CohenA, MassetM, BuckinghamM and FrezalJ. (1989). Chromosomal assignment of 2 myosin alkali light-chain genes encoding the ventricular slow skeletal-muscle isoform and the atrial fetal muscle isoform (Myl3, Myl4). Hum Genet, 81:278–282.
75.
BiressiS, TagliaficoE, LamorteG, MonteverdeS, TenediniE, RoncagliaE, FerrariS, FerrariS, AngelisMGCD, TajbakhshS and CossuG. (2007). Intrinsic phenotypic diversity of embryonic and fetal myoblasts is revealed by genome-wide gene expression analysis on purified cells. Dev Biol, 304:633–651.
ColasAR, McKeithanWL, CunninghamTJ, BushwayPJ, GarmireLX, DuesterG, SubramaniamS and MercolaM. (2012). Whole-genome microRNA screening identifies let-7 and mir-18 as regulators of germ layer formation during early embryogenesis. Genes Dev, 26:2567–2579.
78.
WangY, BaskervilleS, ShenoyA, BabiarzJE, BaehnerL and BlellochR. (2008). Embryonic stem cell-specific microRNAs regulate the G1-S transition and promote rapid proliferation. Nat Genet, 40:1478–1483.
79.
MeltonC, JudsonRL and BlellochR. (2010). Opposing microRNA families regulate self-renewal in mouse embryonic stem cells. Nature, 463:621–626.
80.
XuN, PapagiannakopoulosT, PanG, ThomsonJA and KosikKS. (2009). MicroRNA-145 regulates OCT4, SOX2, and KLF4 and represses pluripotency in human embryonic stem cells. Cell, 137:647–658.
81.
NaguibnevaI, Ameyar-ZazouaM, PolesskayaA, Ait-Si-AliS, GroismanR, SouidiM, CuvellierS and Harel-BellanA. (2006). The microRNA miR-181 targets the homeobox protein Hox-A11 during mammalian myoblast differentiation. Nat Cell Biol, 8:278–284.
82.
ChenJF, MandelEM, ThomsonJM, WuQ, CallisTE, HammondSM, ConlonFL and WangDZ. (2006). The role of microRNA-1 and microRNA-133 in skeletal muscle proliferation and differentiation. Nat Genet, 38:228–233.
83.
ZhangD, LiX, ChenC, LiY, ZhaoL, JingY, LiuW, WangX, ZhangY, et al. (2012). Attenuation of p38-mediated miR-1/133 expression facilitates myoblast proliferation during the early stage of muscle regeneration. PloS One, 7:e41478.
84.
KanoM, SekiN, KikkawaN, FujimuraL, HoshinoI, AkutsuY, ChiyomaruT, EnokidaH, NakagawaM and MatsubaraH. (2010). miR-145, miR-133a and miR-133b: tumor-suppressive miRNAs target FSCN1 in esophageal squamous cell carcinoma. Int J Cancer, 127:2804–2814.
85.
Kassar-DuchossoyL, GiaconeE, Gayraud-MorelB, JoryA, GomesD and TajbakhshS. (2005). Pax3/Pax7 mark a novel population of primitive myogenic cells during development. Genes Dev, 19:1426–1431.
86.
MontarrasD, MorganJ, CollinsC, RelaixF, ZaffranS, CumanoA, PartridgeT and BuckinghamM. (2005). Direct isolation of satellite cells for skeletal muscle regeneration. Science, 309:2064–2067.
87.
BoutetSC, CheungTH, QuachNL, LiuL, PrescottSL, EdalatiA, IoriK and RandoTA. (2012). Alternative polyadenylation mediates microRNA regulation of muscle stem cell function. Cell Stem Cell, 10:327–336.
88.
HorstD, UstaninaS, SergiC, MikuzG, JuergensH, BraunT and VorobyovE. (2006). Comparative expression analysis of Pax3 and Pax7 during mouse myogenesis. Int J Dev Biol, 50:47–54.
89.
DekelI, MagalY, Pearson-WhiteS, EmersonCP and ShaniM. (1992). Conditional conversion of ES cells to skeletal muscle by an exogenous MyoD1 gene. New Biol, 4:217–224.
90.
ShaniM, FaermanA, EmersonCP, Pearson-WhiteS, DekelI and MagalY. (1992). The consequences of a constitutive expression of MyoD1 in ES cells and mouse embryos. Symp Soc Exp Biol, 46:19–36.
91.
ArmourC, GarsonK and McBurneyMW. (1999). Cell-cell interaction modulates myoD-induced skeletal myogenesis of pluripotent P19 cells in vitro. Exp Cell Res, 251:79–91.
92.
GianakopoulosPJ, MehtaV, VoronovaA, CaoY, YaoZ, CoutuJ, WangX, WaddingtonMS, TapscottSJ and SkerjancIS. (2011). MyoD directly up-regulates premyogenic mesoderm factors during induction of skeletal myogenesis in stem cells. J Biol Chem, 286:2517–2525.
93.
CraftAM, KriskyDM, WechuckJB, LobenhoferEK, JiangY, WolfeDP and GloriosoJC. (2008). Herpes simplex virus-mediated expression of Pax3 and MyoD in embryoid bodies results in lineage-Related alterations in gene expression profiles. Stem Cells, 26:3119–3129.
94.
DarabiR, SantosFN, FilaretoA, PanW, KoeneR, RudnickiMA, KybaM and PerlingeiroRC. (2011). Assessment of the myogenic stem cell compartment following transplantation of pax3/pax7-induced embryonic stem cell-derived progenitors. Stem Cells, 29:777–790.
95.
DarabiR, PanW, BosnakovskiD, BaikJ, KybaM and PerlingeiroRC. (2011). Functional myogenic engraftment from mouse iPS cells. Stem Cell Rev, 7:948–957.
96.
DarabiR, SantosFN and PerlingeiroRC. (2008). The therapeutic potential of embryonic and adult stem cells for skeletal muscle regeneration. Stem Cell Rev, 4:217–225.
DarabiR, ArpkeRW, IrionS, DimosJT, GrskovicM, KybaM and PerlingeiroRCR. (2012). Human ES- and iPS-Derived Myogenic Progenitors Restore DYSTROPHIN and Improve Contractility upon Transplantation in Dystrophic Mice. Cell Stem Cell, 10:610–619.
99.
BohelerKR, CzyzJ, TweedieD, YangHT, AnisimovSV and WobusAM. (2002). Differentiation of pluripotent embryonic stem cells into cardiomyocytes. Circ Res, 91:189–201.
100.
BuckinghamM and VincentSD. (2009). Distinct and dynamic myogenic populations in the vertebrate embryo. Curr Opin Genet Dev, 19:444–453.
101.
LiX, ZhangJ, GaoL, McClellanS, FinanMA, ButlerTW, OwenLB, PiazzaGA and XiY. (2011). MiR-181 mediates cell differentiation by interrupting the Lin28 and let-7 feedback circuit. Cell Death Differ, 19:378–386.
102.
HiraiH, VermaM, WatanabeS, TastadC, AsakuraY and AsakuraA. (2010). MyoD regulates apoptosis of myoblasts through microRNA-mediated down-regulation of Pax3. J Cell Biol, 191:347–365.
RohwedelJ, MaltsevV, BoberE, ArnoldHH, HeschelerJ and WobusAM. (1994). Muscle cell differentiation of embryonic stem cells reflects myogenesis in vivo: developmentally regulated expression of myogenic determination genes and functional expression of ionic currents. Dev Biol, 164:87–101.
105.
NewmanAM and CooperJB. (2010). Lab-specific gene expression signatures in pluripotent stem cells. Cell Stem Cell, 7:258–262.
106.
BockC, KiskinisE, VerstappenG, GuH, BoultingG, SmithZD, ZillerM, CroftGF, AmorosoMW, et al. (2011). Reference Maps of human ES and iPS cell variation enable high-throughput characterization of pluripotent cell lines. Cell, 144:439–452.
107.
HumpherysD, EgganK, AkutsuH, HochedlingerK, RideoutWM3rd, BiniszkiewiczD, YanagimachiR and JaenischR. (2001). Epigenetic instability in ES cells and cloned mice. Science, 293:95–97.
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