Human parthenogenetic embryonic stem cells (hpESCs) established from artificially activated oocytes have a wider immune-matching ability because of their homozygosity in the major histocompatibility complex alleles. Whether these cells possess the differentiation capacity similar to regular human embryonic stem cells (hESCs) derived from fertilized eggs is unclear. The aims of this study were to determine whether hpESCs could be differentiated into multipotent mesenchymal stem cell (MSC)-like cells in vitro and then compare these cells with those derived from hESCs. MSC-like cells were obtained from both hpESCs and hESCs, which exhibited similar cell surface marker expression profiles. Further analyses revealed that cells derived from hpESCs possessed stronger osteogenic but weaker adipogenic potentials compared with cells derived from hESCs. This is the first work that demonstrates the differentiation of hpESCs into multipotent MSC-like cells. These hpESCs could be a potential source for cell-based therapies.
Get full access to this article
View all access options for this article.
References
1.
ThomsonJA, Itskovitz-EldorJ, ShapiroSS, WaknitzMA, SwiergielJJ, MarshallVS, JonesJM. 1998. Embryonic stem cell lines derived from human blastocysts. Science, 282:1145–1147.
2.
LinG, OuYangQ, ZhouX, GuY, YuanD, LiW, LiuG, LiuT, LuG. 2007. A highly homozygous and parthenogenetic human embryonic stem cell line derived from a one-pronuclear oocyte following in vitro fertilization procedure. Cell Res, 17:999–1007.
3.
MaiQ, YuY, LiT, WangL, ChenMJ, HuangSZ, ZhouC, ZhouQ. 2007. Derivation of human embryonic stem cell lines from parthenogenetic blastocysts. Cell Res, 17:1008–1019.
MannJR, Lovell-BadgeRH. 1984. Inviability of parthenogenones is determined by pronuclei, not egg cytoplasm. Nature, 310:66–67.
10.
AllenND, BartonSC, HiltonK, NorrisML, SuraniMA. 1994. A functional analysis of imprinting in parthenogenetic embryonic stem cells. Development, 120:1473–1482.
11.
PoradaCD, ZanjaniED, Almeida-PoradG. 2006. Adult mesenchymal stem cells: a pluripotent population with multiple applications. Curr Stem Cell Res Ther, 1:365–369.
12.
CaplanAI. 2007. Adult mesenchymal stem cells for tissue engineering versus regenerative medicine. J Cell Physiol, 213:341–347.
13.
MotalnH, SchichorC, LahTT. 2010. Human mesenchymal stem cells and their use in cell-based therapies. Cancer, 116:2519–2530.
HwangNS, VargheseS, LeeHJ, ZhangZ, YeZ, BaeJ, ChengL, ElisseeffJ. 2008. In vivo commitment and functional tissue regeneration using human embryonic stem cell-derived mesenchymal cells. Proc Natl Acad Sci U S A, 105:20641–20646.
17.
OlivierEN, RybickiAC, BouhassiraEE. 2006. Differentiation of human embryonic stem cells into bipotent mesenchymal stem cells. Stem Cells, 24:1914–1922.
18.
MahmoodA, HarknessL, SchroderHD, AbdallahBM, KassemM. 2010. Enhanced differentiation of human embryonic stem cells to mesenchymal progenitors by inhibition of TGF-beta/activin/nodal signaling using SB-431542. J Bone Miner Res, 25:1216–1233.
19.
de PeppoGM, SvenssonS, LennerasM, SynnergrenJ, StenbergJ, StrehlR, HyllnerJ, ThomsenP, KarlssonC. 2010. Human embryonic mesodermal progenitors highly resemble human mesenchymal stem cells and display high potential for tissue engineering applications. Tissue Eng Part A, 16:2161–2182.
20.
DenisJA, Rochon-BeaucourtC, ChamponB, PietuG. 2011. Global transcriptional profiling of neural and mesenchymal progenitors derived from human embryonic stem cells reveals alternative developmental signaling pathways. Stem Cells Dev, 2011[Epub ahead of print].
21.
MateizelI, De BeckerA, Van de VeldeH, De RyckeM, Van SteirteghemA, CornelissenR, Van der ElstJ, LiebaersI, Van RietI, SermonK. 2008. Efficient differentiation of human embryonic stem cells into a homogeneous population of osteoprogenitor-like cells. Reprod Biomed Online, 16:741–753.
22.
WuCF, TsungHC, ZhangWJ, WangY, LuJH, TangZY, KuangYP, JinW, CuiL, LiuW, CaoYL. 2005. Improved cryopreservation of human embryonic stem cells with trehalose. Reprod Biomed Online, 11:733–739.
23.
ChenFG, ZhangWJ, BiD, LiuW, WeiX, ChenFF, ZhuL, CuiL, CaoY. 2007. Clonal analysis of nestin(-) vimentin(+) multipotent fibroblasts isolated from human dermis. J Cell Sci, 120:2875–2883.
24.
LiuQ, CenL, YinS, ChenL, LiuG, ChangJ, CuiL. 2008. A comparative study of proliferation and osteogenic differentiation of adipose-derived stem cells on akermanite and beta-TCP ceramics. Biomaterials, 29:4792–4799.
NaritaY, YamawakiA, KagamiH, UedaM, UedaY. 2008. Effects of transforming growth factor-beta 1 and ascorbic acid on differentiation of human bone-marrow-derived mesenchymal stem cells into smooth muscle cell lineage. Cell Tissue Res, 333:449–459.
27.
BarberiT, WillisLM, SocciND, StuderL. 2005. Derivation of multipotent mesenchymal precursors from human embryonic stem cells. PLoS Med, 2:e161.
28.
LianQ, LyeE, Suan YeoK, Khia Way TanE, Salto-TellezM, LiuTM, PalanisamyN, El OakleyRM, LeeEH, LimB, LimSK. 2007. Derivation of clinically compliant MSCs from CD105+, CD24− differentiated human ESCs. Stem Cells, 25:425–436.
29.
HemattiP. 2010. Human embryonic stem cell-derived mesenchymal progenitors: an overview. Methods Mol Biol, 690:163–174.
30.
KodairaK, ImadaM, GotoM, TomoyasuA, FukudaT, KamijoR, SudaT, HigashioK, KatagiriT. 2006. Purification and identification of a BMP-like factor from bovine serum. Biochem Biophys Res Commun, 345:1224–1231.
31.
CzyzJ, WobusA. 2001. Embryonic stem cell differentiation: the role of extracellular factors. Differentiation, 68:167–174.
32.
AllegrucciC, YoungLE. 2007. Differences between human embryonic stem cell lines. Hum Reprod Update, 13:103–120.
33.
Le BlancK, TammikL, SundbergB, HaynesworthSE, RingdenO. 2003. Mesenchymal stem cells inhibit and stimulate mixed lymphocyte cultures and mitogenic responses independently of the major histocompatibility complex. Scand J Immunol, 57:11–20.
34.
BuravkovaLB, AndreevaER. 2010. Interaction of human multipotential mesenchymal stromal and immune cells. Fiziol Cheloveka, 36:110–120.
Supplementary Material
Please find the following supplemental material available below.
For Open Access articles published under a Creative Commons License, all supplemental material carries the same license as the article it is associated with.
For non-Open Access articles published, all supplemental material carries a non-exclusive license, and permission requests for re-use of supplemental material or any part of supplemental material shall be sent directly to the copyright owner as specified in the copyright notice associated with the article.
