Stem Cell Mobilization in Normal Donors

Abstract

We studied peripheral blood and apheresis samples from 39 consecutive normal donors who were parents or siblings of patients who received matched or mismatched bone marrow transplants using a combination of rhG-CSF-mobilized peripheral blood stem cells (PBSCs) and bone marrow (BM). BM was harvested from donors 1–7 days before starting rhG-CSF treatment: 12 μg/kg/day rhG-CSF was administered by continuous s.c. infusion for 4–7 days. Peripheral blood progenitor cells were harvested by leukapheresis using an automated continuous-flow blood cell separator, beginning on day 4 of rhG/CSF, for 1–4 consecutive days. Peak peripheral blood CD34+ cell and CFU-GM levels were reached simultaneously on day 5 or 6 of rhG-CSF administration. Median peak levels were 1.65% for CD34+ cells (range 0.34%–4.7%) and 142 CFU-GM/10⁵ plated cells (range 16–700). The greatest numbers of CD34+ cells and CFU-GM, expressed per liter of blood volume processed, were harvested during the second and third leukaphereses: CD34+ cells 37.77 ± 25.48 × 10⁶ and CFU-GM 3.32 ± 2.51 × 10⁶ during the second leukapheresis, and CD34+ cells 37.01 ± 16.33 × 10⁶ and CFU-GM 3.82 ± 4.36 × 10⁶ during the third. The number of CD34+ cells and CFU-GM did not correlate with the sex, age, or body weight of the donors. This study indicates that this protocol for administration of rhG-CSF mobilizes large numbers of hematopoietic progenitor cells into the peripheral blood and that bone marrow harvesting before G-CSF administration does not impair stem cell mobilization.