Isolation of Human CD34 + Peripheral Blood Stem Cells Using an Immunomagnetic Positive Selection System: Prior Monocyte Depletion Using a Nylon-Wool Column

Abstract

An immunomagnetic separation system has been used to collect CD34⁺ cells in mobilized blood after treatment with a nylon-wool column. Cell purities were increased from 2.6% preseparation to 94.6% postseparation, with a mean yield 45.2% (n = 4). Forty percent of CD34⁺ cells separated by the immunomagnetic procedure formed colonies in the presence of hematopoietic growth factors in a limiting dilution assay. Eleven percent of these clones proliferated to over 10⁵ cells and contained megakaryocytes.