Abstract
Prior to purging, cryopreservation, or ABO-incompatible bone marrow (BM) transplantation, we have concentrated 23 BM harvests using a modification of the "grancollect-protocol" and the recently available bone marrow stem cell (BMSC) protocol of the Fresenius AS 104 cell separator with the P1-Y set. Within 40-70 minutes, the initial marrow volume of 922 ml (±408 ml) was processed two to three times. A mean of 59% (±20%) of the initial mononuclear cells was recovered in a mean volume of 119 ml (±31 ml). The recovery of clonogenic cells, measured by CFU-GM assays, was 98% (±80%). Red blood cells in the BM concentrates were reduced to 8% (±4%) of the initial number. The procedure was efficient and yielded a BM cell fraction suitable for purging, cryopreservation, and transplantation. All transplanted patients showed fast and sustained engraftments after autologous or allogeneic BM transplantation.
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