This study was designed to explore how supercritical fluid CO2 extract of Ligusticum chuanxiong Hort (CX) protects mouse liver and kidney from d-galactose-induced injury. The antioxidant capacity of CX was confirmed both in vitro and in vivo. The d-galactose-induced malondialdehyde increase was attenuated by CX, as well as the increase in aspartate aminotransferase, alanine aminotransferase, blood urea nitrogen, and creatinine level. In addition, the activities of antioxidant enzymes were markedly renewed, and the gene expressions of these enzymes were upregulated in CX groups. The results of histological analysis suggested that CX could effectively attenuate the d-galactose-induced structure damage. Furthermore, results of Western blotting analysis showed that CX significantly inhibited the upregulation of nuclear factor protein expression caused by d-galactose. In conclusion, CX could attenuate the liver and kidney injury in d-galactose-treated mice, and the mechanism might be associated with attenuating oxidative stress and inflammatory response.
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References
1.
UfukC, SevalA, PınarA, KarolinY, MustafaES, EnisD, et al.increased protein oxidation and loss of protein-bound sialic acid in hepatic tissues of D-galactose induced aged rats. Curr Aging Sci, 2013; 6:135–141.
2.
AydınS, YanarK, AtukerenP, DaloE, SitarME, UsluE, et al.Comparison of oxidative stress biomarkers in renal tissues of D-galactose induced, naturally aged and young rats. Biogerontology, 2012; 13:251–260.
3.
LeeS-J, LimK-T. UDN glycoprotein regulates activities of manganese-superoxide dismutase, activator protein-1, and nuclear factor-κB stimulated by reactive oxygen radicals in lipopolysaccharide-stimulated HCT-116 cells. Cancer Lett, 2007; 254:274–287.
4.
SarkarD, SahaP, GamreS, BhattacharjeeS, HariharanC, GangulyS, et al.Anti-inflammatory effect of allylpyrocatechol in LPS-induced macrophages is mediated by suppression of iNOS and COX-2 via the NF-κB pathway. Int Immunopharmacol, 2008; 8:1264–1271.
5.
LeeS-J, LimK-T. Glycoprotein isolated from Ulmus davidiana Nakai regulates expression of iNOS and COX-2 in vivo and in vitro. Food Chem Toxicol, 2007; 45:990–1000.
6.
ZhangZ-F, FanS-H, ZhengY-L, LuJ, WuD-M, ShanQ, et al.Purple sweet potato color attenuates oxidative stress and inflammatory response induced by d-galactose in mouse liver. Food Chem Toxicol, 2009; 47:496–501.
7.
FanS-H, ZhangZ-F, ZhengY-L, LuJ, WuD-M, ShanQ, et al.Troxerutin protects the mouse kidney from d-galactose-caused injury through anti-inflammation and anti-oxidation. Int Immunopharmacol, 2009; 9:91–96.
8.
SamojlikI, LakićN, Mimica-DukićN, Đaković-ŠvajcerK, BožinB. Antioxidant and hepatoprotective potential of essential oils of coriander (Coriandrum sativum L.) and Caraway (Carum carvi L.) (Apiaceae). J Agric Food Chem, 2010; 58:8848–8853.
9.
LiuC-M, MaJ-Q, LouY. Chronic administration of troxerutin protects mouse kidney against d-galactose-induced oxidative DNA damage. Food Chem Toxicol, 2010; 48:2809–2817.
10.
DambachDM, DurhamSK, LaskinJD, LaskinDL. Distinct roles of NF-κB p50 in the regulation of acetaminophen-induced inflammatory mediator production and hepatotoxicity. Toxicol Appl Pharmacol, 2006; 211:157–165.
11.
LiuJ-L, ZhengS-L, FanQ-J, YuanJ-C, YangS-M, KongF-L. Optimization of high-pressure ultrasonic-assisted simultaneous extraction of six major constituents from Ligusticum chuanxiong Rhizome using response surface methodology. Molecules, 2014; 19:1887.
12.
HouY-Z, ZhaoG-R, YuanY-J, ZhuG-G, HiltunenR. Inhibition of rat vascular smooth muscle cell proliferation by extract of Ligusticum chuanxiong and Angelica sinensis. J Ethnopharmacol, 2005; 100:140–144.
13.
KimEY, RhyuMR. Synergistic vasorelaxant and antihypertensive effects of Ligusticum wallichii and Angelica gigas. J Ethnopharmacol, 2010; 130:545–551.
14.
LiCM, WuJH, YangRF, DongXL, HeZY, TianXL, et al.Ligusticum chuanxiong prevents ovariectomy-induced liver and vascular damage in rats. Am J Chin Med, 2013; 41:831–848.
15.
JeongJB, JuSY, ParkJH, LeeJR, YunKW, KwonST, et al.Antioxidant activity in essential oils of Cnidium officinale makino and Ligusticum chuanxiong Hort and their inhibitory effects on DNA damage and apoptosis induced by ultraviolet B in mammalian cell. Cancer Epidemiol, 2009; 33:41–46.
16.
HouYZ, ZhaoGR, YangJ, YuanYJ, ZhuGG, HiltunenR. Protective effect of Ligusticum chuanxiong and Angelica sinensis on endothelial cell damage induced by hydrogen peroxide. Life Sci, 2004; 75:1775–1786.
17.
LiJ-J, ZhuQ, LuY-P, ZhaoP, FengZ-B, QianZ-M, et al.Ligustilide prevents cognitive impairment and attenuates neurotoxicity in d-galactose induced aging mice brain. Brain Res, 2015; 1595:19–28.
18.
YangBC, JinLL, YangYF, LiK, PengDM. Inhibitory effect of rape pollen supercritical CO2 fluid extract against testosterone-induced benign prostatic hyperplasia in rats. Exp Ther Med, 2014; 8:31–37.
19.
ChenQ, LiP, LiB, YangH, LiX, ChenF. A GC–MS-SIM simultaneous determination of ligustilide and butylidenephthalide from Ligusticum chuanxiong using SFE. Chromatographia, 2010; 72:963–967.
20.
ZhangH, HanT, YuC-H, JiangY-P, PengC, RanX, et al.Analysis of the chemical composition, acute toxicity and skin sensitivity of essential oil from rhizomes of Ligusticum chuanxiong. J Ethnopharmacol, 2012; 144:791–796.
21.
KaragozlerAA, ErdayB, EmekYC, UggunDA. Antioxidant activity and proline content of leaf extracts from Dorystoechas hastata. Food Chem, 2008; 111:400–407.
22.
VijayabaskarP, ShiyamalaV. Antioxidant properties of seaweed polyphenol from Turbinaria ornata (Turner) J Agardh. Asian Pac J Trop Biomed, 2012; 2:S90–S98.
23.
NishimikiM, RaoNA, YagiK. The occurrence of superoxide anion in the reaction of reduced phenazine methosulfate and molecular oxygen. Biochem Biophys Res Commun, 1972; 46:849–853.
24.
LimYY, LimTT, TeeJJ. Antioxidant properties of several tropical fruits: A comparative study. Food Chem, 2007; 103:1003–1008.
25.
MiharaM, UchiyamaM. Determination of malonaldehyde precursor in tissues by thiobarbituric acid test. Anal Biochem, 1978; 86:271–278.
26.
PagliaDE, ValentineWN. Studies on the quantitative and qualitative characterization of erythrocyte glutathione peroxidase. J Lab Clin Med, 1967; 70:158–169.
27.
ChenB, ZhongY, PengW, SunY, KongWJ. Age-related changes in the central auditory system: Comparison of D-galactose-induced aging rats and naturally aging rats. Brain Res, 2010; 1344:43–53.
28.
CebeT, AtukerenP, YanarK, KuruçAI, OzanT, KunbazA, et al.Oxidation scrutiny in persuaded aging and chronological aging at systemic redox homeostasis level. Exp Gerontol, 2014; 57:132–140.
29.
CebeT, YanarK, AtukerenP, OzanT, KuruçAI, KunbazA, et al.A comprehensive study of myocardial redox homeostasis in naturally and mimetically aged rats. Age (Dordr), 2014; 36:9728.
30.
UzunD, KorkmazGG, et al.Oxidative damage parameters in renal tissues of aged and young rats based on gender. Clin Interv Aging, 2013; 8:809–815.
31.
UweS. Anti-inflammatory interventions of NF-κB signaling: Potential applications and risks. Biochem Pharmacol, 2008; 75:1567–1579.
32.
Müller-DeckerK, BergerI, AckermannK, EhemannV, ZoubovaS, AulmannS, et al.Cystic duct dilatations and proliferative epithelial lesions in mouse mammary glands upon keratin 5 promoter-driven overexpression of cyclooxygenase-2. Am J Pathol, 2005; 166:575–584.
