Abstract
In erythrocytes, although three amino acids are required for the synthesis of reduced glutathione (GSH), the rate of GSH synthesis is determined only by the availability of L-cysteine. Cysteine supplementation has been shown to ameliorate several parameters that are known to degenerate during human aging; this has led to an interesting hypothesis that aging could be a cysteine deficiency syndrome. In the present study, we measured L-cysteine influx in human erythrocytes by suspending cells in solution containing 10 mM L-cysteine. We show a significant decline in the influx of L-cysteine in erythrocytes during aging in humans. The decrease in cysteine influx correlates with the decrease in antioxidant potential of plasma measured in terms of FRAP (ferric-reducing ability of plasma) during aging. We conclude that a decreased influx of L-cysteine may be an important factor contributing to the development of oxidative stress in human erythrocytes during aging.
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