The search for alternative therapeutics against antibiotic-resistant bacteria is highly desirable. A promising approach is photodynamic antimicrobial chemotherapy.
Objective:
This work evaluated the photodynamic inactivation (PDI) efficacy of hypocrellin B (HB) on Gram-positive antibiotic-resistant bacteria.
Methods:
PDI efficacy of HB on Gram-positive standard and antibiotic-resistant Staphylococcus aureus, Enterococcus faecalis, and Streptococcus pneumonia and Gram-negative Escherichia coli and Klebsiella pneumoniae was assessed. HB photoactivity on biofilms formed by the Gram-positive bacteria and its cytotoxicity on mammalian CT26 cells were also investigated.
Results:
HB showed no obvious dark toxicity, but provided concentration-dependent inactivation of bacteria and mammalian cells. After irradiation with 72 J/cm2 light, 100 μM of HB achieved about 7 log10 reductions in bacterial survival of Gram-positive strains, but yielded only 2 log10 reductions in bacterial survival of Gram-negative strains. Gram-positive bacteria were as susceptible to PDI in biofilms as in planktonic suspensions, but the efficacy was attenuated.
Conclusions:
The results suggested that HB could serve as a potential antibacterial photosensitizer against Gram-positive antibiotic-resistant bacteria.
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References
1.
HamblinMR, HasanT. Photodynamic therapy: a new antimicrobial approach to infectious disease. Photochem Photobiol Sci, 2004; 3:436–450.
2.
St. DenisTG, DaiT, IziksonL, et al.All you need is light, antimicrobial photoinactivation as an evolving and emerging discovery strategy against infectious disease. Virulence, 2011; 2:509–520.
3.
NicolauDP. Current challenges in the management of the infected patient. Curr Opin Infect Dis, 2011; 24:1–10.
4.
SchastakS, SvitlanaZ, GitterB, WiedermannP, ClaudepierreT. Efficient photodynamic therapy against gram-positive and gram-negative bacteria using THPTS, a cationic photosensitizer excited by infrared wavelength. PLoS One, 2010; 5:e11674.
5.
LiuY, QinR, SebastianAJ, et al.Antibacterial photodynamic therapy: overview of a promising approach to fight antibiotic-resistant bacterial infections. J Clin Transl Res, 2015; 1:140–167.
6.
RinehA, BremnerJB, HamblinMR, et al.Attaching the NorA efflux pump inhibitor INF55 to methylene blue enhances antimicrobial photodynamic inactivation of methicillin-resistant Staphylococcus aureusin vitro and in vivo. ACS Infect Dis, 2017; 3:756–766.
JoriG, FabrisC, SoncinM, et al.Photodynamic therapy in the treatment of microbial infections: basic principles and perspective applications. Laser Surg Med, 2006; 38:468–481.
9.
MaischT. Resistance in antimicrobial photodynamic inactivation of bacteria. Photochem Photobiol Sci, 2015; 14:1518–1526.
10.
BrancoTM, ValerionNC, JesusVIC, et al.Single and combined effects of photodynamic therapy and antibiotics to inactivate Staphylococcus aureus on skin. Photodiagnosis Photodyn Ther2018; 21:285–293.
11.
KrishnaswamiV, PonnusamyC, SankareswaranS, et al.Development of copolymeric nanoparticles of hypocrellin B: enhanced phototoxic effect and ocular distribution. Eur J Pharm Sci, 2018; 116:26–36.
12.
ZhaoH, YinR, ChenD, et al.In vitro and in vivo antitumor activity of a novel hypocrellin B derivative for photodynamic therapy. Photodiagnosis Photodyn Ther, 2014; 112:204–212.
13.
JiangY, LeungAW, XiangJ, XuC. LED light-activated hypocrellin B induces mitochondrial damage of ovarian cancer cells. Int J Photoenergy, 2012; 186752.
14.
JiangY, LeungAW, WangXN, ZhangHW, XuCS. Effect of photodynamic therapy with hypocrellin B on apoptosis, adhesion, and migration of cancer cells. Int J Radiat Biol, 2014; 90:575579.
15.
ZhaoK, JiangL. Conversion of hypocrellin A in alkaline and neutral media. Chin J Org Chem, 1989; 9:252–254.
16.
PengZ, TuB, ShenY, et al.Quaternized chitosan inhibits icaA transcription and biofilm formation by Staphylococcus on a titanium surface. Antimicrob Agents Chemother, 2011; 55:860–866.
17.
MuluyeRA, BianY, AlemuPN. Anti-inflammatory and antimicrobial effects of heat-clearing Chinese herbs: a current review. J Tradit Complement Med, 2014; 4:93–98.
18.
QiF. Traditional Chinese medicine and related active compounds: a review of their role on hepatitis B virus infection. Drug Discov Ther, 2013; 7:212–224.
19.
YuC. Encyclopedia of Chinese Medicines, Vol. 1. Beijng, P.R. China: Chinese Medicinal Science and Technology Press, 1993, p. 1187.
20.
MaG, KhanSI, JacobMR, et al.Antimicrobial and antileishmanial activities of hypocrellins A and B. Antimicrob Agents Chemother, 2004; 48:4450–4452.
21.
HuY, ZhangC, LiS, et al.Effects of photodynamic therapy using yellow led-light with concomitant hypocrellin B on apoptotic signaling in keloid fibroblasts. Int J Biol Sci, 2015; 13:319–326.
22.
JiangY, LeungAW, WangX, ZhangH, XuC. Inactivation of Staphylococcus aureus by photodynamic action of hypocrellin B. Photodiagnosis Photodyn Ther, 2013; 10:600–606.
23.
MahTFC, O'TooleGA. Mechanisms of biofilm resistance to antimicrobial agents. Trends Microbiol, 2001; 9:34–39.
24.
PereiraMA, FaustinoMA, TomeJP, et al.Influence of external bacterial structures in the efficiency of photodynamic inactivation by a cationic porphyrin. Photochem Photobiol Sci, 2014; 13:680–690.
25.
LiT, HouX, DengH, et al.Liposomal hypocrellin B as a potential photosensitizer for age-related macular degeneration: pharmacokinetics, photodynamic efficacy, and skin phototoxicity in vivo. Photochem Photobiol Sci, 2015; 14:972–981.
26.
ChangJE, ChoHY, YiE, KimDD, JheonS. Hypocrellin B and paclitaxel-encapsulated hyaluronic acid-ceramide nanoparticles for targeted photodynamic therapy in lung cancer. J Photochem Photobiol B, 2016; 158:113–121.
27.
JiangY, PangX, LiuR, et al.Design of an amphiphilic iRGD peptide and self-assembling nanovesicles for improving tumor accumulation and penetration and the photodynamic efficacy of the photosensitizer. ACS Appl Mater Interfaces, 2018; 10:31674–31685.
28.
HamblinMR. Antimicrobial photodynamic inactivation: a bright new technique to kill resistant microbes. Curr Opin Microbiol, 2016; 33:67–73.
29.
HuangL, DaiT, HamblinMR. Antimicrobial photodynamic inactivation and photodynamic therapy for infections. Methods Mol Biol, 2010; 635:155–173.
30.
DaiT, TegosGP, ZhiyentayevT, et al.Photodynamic therapy for methicillin-resistant Staphylococcus aureus infection in a mouse skin abrasion model. Lasers Srug Med, 2010; 42:38.
31.
ZhouZ, PengS, SuiM, et al.Multifunctional nanocomplex for surface-enhanced Raman scatteringimaging and near-infrared photodynamic antimicrobial therapy of vancomycin-resistant bacteria. Colloids Surf B, 2018; 161:394–402.
