The purpose of this in vitro study was to evaluate the antimicrobial effect of activated irrigation with different modes of erbium-doped yttrium aluminum garnet (Er:YAG) laser application on microorganisms related to secondary endodontic infection.
Background:
Er:YAG laser has been recommended as an adjuvant tool for root canal disinfection during endodontic treatment.
Materials and methods:
Laser-activated irrigation (LAI) with 300 or 600 μm tips were tested with or without intermittent irrigation with 0.9% sodium chloride (NaCl) solution against different microorganisms (five single strains and dual species (Streptococcus gordonii combined with Actinomyces oris or Fusobacterium nucleatum) in root canals after 3 days of incubation. In a 21-day infection model, LAI was used together with intermittent rinsing with sodium hypochlorite (NaOCl) against the dual-species mixtures; here the incidence of microbial regrowth after up to 7 days was monitored.
Results:
In the 3-day root infection model, LAI protocols did not show any significant reduction of the microbial load when compared with manual irrigation with saline solution. In the 21-day infection, S. gordonii combined with A. oris were not detectable anymore after applying the LAI protocol with a 600 μm tip (30 mJ/10 pps) up to 7 days after treatment.
Conclusions:
Application of LAI with a 600 μm tip by using an Er:YAG laser might be advantageous in treatment of endodontic infections.
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