Objective: Laser technologies have many different applications in medical, agricultural, and industrial fields. Studies have shown several effects of laser energy on different bacterial species, in a wide variety of settings. Recent reports have found that one of the unique features of bacteria is their ability to communicate among themselves (quorum sensing). We sought to investigate whether low-energy laser irradiation affects bacterial behavior, which is regulated by quorum sensing. Methods: Laser irradiations were performed using Er:YAG laser (2940 nm wavelength) at output powers of 0.5, 1.5, 2.5, and 4 W on wild-type Vibrio harveyi. Bioluminescence, motility, and biofilm forming capability were assessed on the bacteria after irradiation. Results: After irradiation of bacteria, positive dose/output power dependencies were found in the bioluminescence omitted from tested experimental groups. Motility of colonies on semi-solid media was inhibited as irradiation output power was increased. However, after irradiation, biomass analysis of biofilm samples showed negligible differences between the irradiated samples and controls. Conclusions: Results indicate the impact of low-energy laser irradiation on bacterial behavior such as quorum sensing and motility, without affecting bacterial growth patterns.
StraightPD, KolterR. Interspecies chemical communication in bacterial development. Annu Rev Microbiol, 2009; 63:99–118.
4.
DaviesDG, ParsekMR, PearsonJP, IglewskiBH, CostertonJW, GreenbergEP. The involvement of cell-to-cell signals in the development of a bacterial biofilm. Science, 1998; 280:295–298.
5.
RezzonicoF, DuffyB. Lack of genomic evidence of AI-2 receptors suggests a non-quorum sensing role for luxS in most bacteria. BMC Microbiol, 2008; 8:154.
6.
BasslerBL, WrightM, SilvermanMR. Multiple signalling systems controlling expression of luminescence in Vibrio harveyi: sequence and function of genes encoding a second sensory pathway. Mol Microbiol, 1994; 13:273–286.
7.
WinzerK, HardieKR, WilliamsP. LuxS and autoinducer-2: their contribution to quorum sensing and metabolism in bacteria. Adv Appl Microbiol, 2003; 53:291.
8.
HenkeJM, BasslerBL. Three parallel quorum-sensing systems regulate gene expression in Vibrio harveyi. J Bacteriol, 2004; 186:6902–6914.
9.
AnetzbergerC, ReigerM, FeketeA, et al.Autoinducers act as biological timers in Vibrio harveyi. PLoS One, 2012; 7:e48310.
10.
BarriosGAF, ZuoR, HashimotoY, YangL, BentleyWE, WoodTK. Autoinducer 2 controls biofilm formation in Escherichia coli through a novel motility quorum-sensing regulator (MqsR, B3022). J Bacteriol, 2006; 188:305–316.
11.
HammerBK, BasslerBL. Quorum sensing controls biofilm formation in Vibrio cholerae. Mol Microbiol, 2003; 50:101–104.
12.
RiceSA, KohKS, QueckSY, LabbateM, LamKW, KjellebergS. Biofilm formation and sloughing in Serratia marcescens are controlled by quorum sensing and nutrient cues. J Bacteriol, 2005; 187:3477–3485.
13.
LiJ, AttilaC, WangL, WoodTK, ValdesJJ, BentleyWE. Quorum sensing in Escherichia coli is signaled by AI-2/LsrR: effects on small RNA and biofilm architecture. J Bacteriol, 2007; 189:6011–6020.
14.
VikramA, JesudhasanPR, JayaprakashaGK, PillaiSD, PatilBS. Citrus limonoids interfere with Vibrio harveyi cell-cell signalling and biofilm formation by modulating the response regulator LuxO. Microbiology, 2011; 157:99–110.
15.
RoyV, MeyerMT, SmithJA, et al.AI-2 analogs and antibiotics: a synergistic approach to reduce bacterial biofilms. Appl Microbiol Biotechnol, 2013; 97:2627–2638.
16.
KadirvelM, FanimarvastiF, ForbesS, et al.Inhibition of quorum sensing and biofilm formation in Vibrio harveyi by 4-fluoro-DPD; a novel potent inhibitor of signalling. Chem Commun, 2014; 50:5000–5002.
17.
HongNT, BaruahK, VanrompayD, BossierP. Characterization of phenotype variations of luminescent and non-luminescent variants of Vibrio harveyi wild type and quorum sensing mutants. J Fish Dis, 2016; 39:317–327.
18.
HibstR, KellerU. Experimental studies of the application of the Er:YAG laser on dental hard substances: I. Measurement of the ablation rate. Lasers Surg Med, 1989; 9:338–344.
19.
HentzerM, GivskovM. Pharmacological inhibition of quorum sensing for the treatment of chronic bacterial infections. J Clini Invest, 2003; 112:1300–1307.
20.
ZhangLH, DongYH. Quorum sensing and signal interference: diverse implications. Mol Microbiol, 2004; 53:1563–1571.
21.
JacksonZ, MeghjiS, MacrobertA, HendersonB, WilsonM. Killing of the yeast and Hyphal forms of candida albicans using a light-activated antimicrobial agent. Lasers Med Sci, 1999; 14:150–157.
22.
KarrerS, SzeimiesRM, ErnstS, AbelsC, BaumlerW, LandthalerM. Photodynamic inactivation of staphylococci with 5-aminolaevulinic acid or photofrin. Lasers Med Sci, 1999; 14:54–61.
23.
ZakariasenKL, DederichDN, TulipJ, DeCosteS, JensenSE, PickardMA. Bactericidal action of carbon dioxide laser radiation in experimental dental root canals. Can J Microbiol, 1986; 32:942–946.
24.
WhittersCJ, MacfarlaneTW, MackenzieD, MoseleyH, StrangR. The bactericidal activity of pulsed Nd-YAG laser radiation in vitro. Lasers Med Sci, 1994; 9:297–303.
AharoniR, BronstheynM, JabbourA, ZaksB, SrebnikM, SteinbergD. Oxazaborolidine derivatives inducing autoinducer-2 signal transduction in Vibrio harveyi. Bioorg Med Chem, 2008; 16:1596–1604.
27.
FeldmanM, WeissEI, OfekI, SteinbergD. Interference of cranberry constituents in cell-cell signaling system of Vibrio harveyi. Curr Microbiol, 2009; 59:469–474.
28.
SoniD, SmoumR, BreuerA, MechoulamR, SteinbergD. Effect of the synthetic cannabinoid HU-210 on quorum sensing and on the production of quorum sensing-mediated virulence factors by Vibrio harveyi. BMC Microbiol, 2015; 15:159.
29.
AssafD, SteinbergD, ShemeshM. Lactose triggers biofilm formation by Streptococcus mutans. Int Dairy J, 2015; 42:51–57.
30.
YangQ, DefoirdtT. Quorum sensing positively regulates flagellar motility in pathogenic Vibrio harveyi. Environ Microbiol, 2015; 17:960–968.
31.
RutherfordST, BasslerBL. Bacterial quorum sensing: its role in virulence and possibilities for its control. Cold Spring Harb Perspect Med, 2012; 2:a012427.
32.
GrandclémentC, TannièresM, MoréraS, DessauxY, FaureD. Quorum quenching: role in nature and applied developments. FEMS Microbiol Rev, 2016; 40:86–116.
33.
LubartR, LipovskiA, NitzanY, FriedmannH. A possible mechanism for the bactericidal effect of visible light. Laser Therapy, 2011; 20:17–22.
Cohen-BerneronJ, SteinbergD, FeatherstoneJD, FeuersteinO. Sustained effects of blue light on Streptococcus mutans in regrown biofilm. Lasers Med Sci, 2016; 31:445–452.
36.
CohenJ, FeatherstoneJD, LeCQ, SteinbergD, FeuersteinO. Effects of CO2 laser irradiation on tooth enamel coated with biofilm. Lasers Surg Med, 2014; 46:216–223.
37.
DanielsLL, QuickendenTI. Does low-intensity He-Ne laser radiation produce a photobiological growth response in Escherichia coli?. Photochem Photobiol, 1994; 60:481–485.
38.
NandakumarK, ShinozakiT, ObikaH. Impact of pulsed Nd: YAG laser on the marine biofilm-forming bacteria Pseudoalteromonas carrageenovora: significance of physiological status. Canadian J Microbiol, 2002; 48:326–332.
39.
NussbaumEL, LilgeL, MazzulliT. Effects of low-level laser therapy (LLLT) of 810 nm upon in vitro growth of bacteria: relevance of irradiance and radiant exposure. J Clin Laser Med Surg, 2003; 21:283–290.
40.
PourzarandianA, WatanabeH, RuwanpuraSMPM, AokiA, IshikawaI. Effect of low-level Er:YAG laser irradiation on cultured human gingival fibroblasts. J Periodontol, 2005; 76:187–193.
41.
WatersCM, BasslerBL. The Vibrio harveyi quorum-sensing system uses shared regulatory components to discriminate between multiple autoinducers. Genes Dev, 2006; 20:2754–2767.
42.
LeeMT, BirdPS, WalshLJ. Photo-activated disinfection of the root canal: a new role for lasers in endodontics. Aust Endod J, 2004; 30:93–98.
43.
PeriasamyS, KolenbranderPE. Mutualistic biofilm communities develop with Porphyromonas gingivalis and initial, early, and late colonizers of enamel. J Bacteriol, 2009; 191:6804–6811.
44.
DadrasS, MohajeraniE, EftekharF, HosseiniM. Different photoresponses of Staphylococcus aureus and Pseudomonas aeruginosa to 514, 532, and 633 nm low level lasers in vitro. Curr Microbiol, 2006; 53:282–286.
45.
DefoirdtT, BoonN, SorgeloosP, VerstraeteW, BossierP. Quorum sensing and quorum quenching in Vibrio harveyi: lessons learned from in vivo work. ISME J, 2008; 2:19–26.
46.
TuKC, BasslerBL. Multiple small RNAs act additively to integrate sensory information and control quorum sensing in Vibrio harveyi. Genes Dev, 2007; 21:221–233.
47.
McCarterLL. Dual flagellar systems enable motility under different circumstances. J Mol Microbiol Biotechnol, 2004; 7:18–29.
48.
AnetzbergerC, PirchT, JungK. Heterogeneity in quorum sensing-regulated bioluminescence of Vibrio harveyi. Mol Microbiol, 2009; 73:267–277.
