Objective: The purpose of this study was to evaluate the influence of intense pulsed light (IPL) irradiation on 8-methoxypsoralen plus ultraviolet-A irradiation (PUVA)-induced senescence of fibroblasts in vitro. Background data: Exposure to PUVA may result in stress-induced senescence in fibroblasts. IPL has been widely used to treat photo-aged skin, but the mechanism is not clear. Methods: The expression of senescence-associated β-galactosidase (SA-β-gal) was determined by histochemical staining, cell viability was assessed via an MTT assay, telomere length was determined by real-time polymerase chain reaction (PCR), and changes in the generation of reactive oxygen species (ROS) were determined by flow cytometry (FCM). Results: In comparison with the control cells, cells irradiated with PUVA and PUVA+IPL showed a general elevation in SA-β-gal activity (p<0.05). The number of SA-β-gal-positive fibroblasts in the PUVA+IPL group was clearly lower than that in the PUVA group (p<0.05). Cell viability showed a time-dependent decrease in both the PUVA and PUVA+IPL groups, in comparison with the viability in the control group, and there was no difference in viability between the PUVA and PUVA+IPL groups. PUVA treatment shortened telomere length and increased the level of ROS, in comparison with the corresponding findings for the control cells (p<0.05), whereas irradiation with IPL after PUVA exposure prevented telomere shortening and decreased the ROS level, in comparison with PUVA treatment only (p<0.05). Conclusions: PUVA treatment induced fibroblast senescence, and irradiation with IPL after PUVA exposure partially rejuvenated the cells, demonstrating a protective effect against PUVA-induced fibroblast senescence.
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