Objective: To evaluate 80-Hz pulsed infrared diode low-level laser therapy (LLLT) for third-degree burn healing in rats. Background Data: Review of the literature indicates that LLLT has a biostimulatory effect on wound healing, although no clear recommendation can be made yet. Methods: Forty-eight male rats were divided into groups 1 and 2. On day 0, three third-degree burns were made on the dorsum of each rat using steam. In group 1, the first burn was exposed to an 80-Hz pulsed 890-nm infrared diode laser at 0.396 J/cm2 three times a week. In group 2, the first burn was exposed to the inactivated laser. In both groups, the second burns were treated with topical application of nitrofurazone 0.2%, and third burns did not receive any treatment and were considered as control burns. Burns were clinically examined. The rats were treated for 8, 12, and 20 days in both groups. On days 8, 2, and 20, microbiological samples of burns were collected and analyzed. Data were analyzed using the chi-square method. Results: The paired Student t-test showed that the wound closure rate of laser-treated burns (17.6 ± 1.86 days) was significantly longer than that of the control burns (19.6 ± 0.81days) (p = 0.007).The number of laser-treated burns that closed was significantly higher than controls. Chi-square tests showed that there was not significant difference between each microorganism (Staphylococcus epidermis, S. aureus and Pseudomonas aeruginosa). Conclusion: LLLT using an 80-Hz pulsed infrared diode laser accelerated third-degree burn healing in rat.
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