Abstract
Background:
Poultry products are the largest food category linked to salmonellosis in Canada. Bacteriophages (phages) have been proposed as a novel antimicrobial in the poultry industry due to their documented ubiquity, efficacy, and safety benefits.
Materials and Methods:
A library of 78 lytic phages was rapidly screened against 50 prominent poultry-associated Salmonella enterica isolates procured from British Columbia, Canada.
Results:
A phage cocktail was successfully formulated using only three sewage-isolated phages (SE4, SE13, and SE20) to achieve broad-spectrum antimicrobial efficacy across all S. enterica serovars. Highly promising phages were also characterized using one-step growth curves and transmission electron microscopy.
Conclusion:
Relative host efficiency is a new agar-based semiquantitative metric developed here for the rapid comparison of different phages against a panel of known bacterial targets.
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Supplementary Material
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