Electrochemical DNA Biosensor for the Detection of Interaction between Di[azino-di(5,6-azafluorene)-κ 2 -NN′] Dibromicoppers and DNA

Abstract

A new Cu(II) complex CuL₂Br² (L = azino-di(5,6-azafluorene)-κ²-NN′) was synthesized, and a new method of electrochemical probe has been proposed for the determination of hepatitis B virus (HBV) based on its interaction with [CuL₂]²⁺. This ligand, containing functional groups, as well as planar aromatic domains, is capable of binding to double-stranded DNA (dsDNA) more efficiently than to single-stranded DNA (ssDNA). Emphasis has been placed on the elucidation of the nature of the interaction by electrochemical techniques. The electroactive [CuL₂]²⁺ could be employed as an electrochemical indicator to detect hybridization events in DNA biosensors. These biosensors have been constructed by immobilization of a probe DNA sequence from HBV onto glassy carbon electrode (GCE). After hybridization with the complementary target sequence, [CuL₂]²⁺ was accumulated within the dsDNA layer. Electrochemical detection was performed by differential pulse voltammetry over the potential range. Using this approach, complementary target sequences of HBV can be quantified over the range of 1.74 × 10₋₉ to 3.45 × 10₋₇ M, with a detection limit of 8.32 × 10₋₁₀ M and a linear correlation coefficient of 0.9936.In addition, this approach is capable of detecting hybridization of complementary sequences containing one or three mismatched bases.