Sequence-Specific Cleavage of Yeast tRNA Phe with Oligonucleotides Conjugated to a Diimidazole Construct

Abstract

Oligonucleotide derivatives conjugated to a chemical construction with two histamine residues imitating the catalytic center of ribonuclease A have been synthesized. In experiments with the conjugates complementary to the 3‵-end and to the variable loop and the T loop of yeast tRNA^Phe, it was shown that the compounds can accomplish sequence-specific cleavage of the target RNA in physiologic conditions.