Purpose: This study was designed to evaluate the effects of a dorzolamide–timolol combination
or dorzolamide on retinal ganglion cell (RGC) density and intraocular pressure (IOP)
in glaucomatous eyes of adult rats.
Methods: Glaucoma was induced in the right eye of adult Wistar rats by episcleral venous
occlusion. One experimental group was administered dorzolamide 2%–timolol 0.5% combination
eye drops, while the other experimental group was administered dorzolamide 2% eye
drops. Control groups had surgery without drug administration. Drug application was initiated
either 2 weeks before surgery (Group A), from the day of surgery (Group B), 2 weeks after
surgery (Group C), or 4 weeks after surgery (Group D). RGCs were labeled by intratectal
Fluorogold injections and counted from flat-mount preparations, and IOP was measured using
Tonopen.
Results: Both dorzolamide–timolol combination and dorzolamide, when applied topically,
significantly reduced IOP and improved RGC densities in experimental eyes when compared
to control eyes. Earlier initiation, as well as longer duration of drug application, resulted in
higher RGC densities.
Conclusions: Topical application of a dorzolamide–timolol combination or dorzolamide
saved RGCs to a significant extent and reduced IOP in glaucomatous rat eyes.
