Abstract
ABSTRACT
We have devised a new method to solubilize sciera in order to permit quantitative determination of tobramycin by commercially available and widely utilized means. The sciera, containing tobramycin, was digested with Alcalase (Subtilisn A, NOVO Enzymes) and the tobramycin level was guantitated by TDX autoflourophotometry. This technique is accurate and sensitive. The tissue digestion and antibiotic assay are relatively simple to perform and should be applicable to a variety of antibiotics and possibly other therapeutic agents.
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