Crataegus songarica is a native species of Pakistan. Its fruits are locally consumed for the treatment of various cardiovascular disorders, including hypertension, heart failure, and vascular insufficiency. Despite its traditional use, data regarding its effectiveness remain unclear. Therefore, the aim of the study was to evaluate the cardioprotective effects of C. songarica fruit extract on glucose-induced hypertensive rats. First, the aqueous-methanol extract (AMECS) was obtained and subjected to phytochemical characterization. The antioxidant and cytotoxic properties were investigated in vitro. Acute toxicity as well as screening for hypotensive effects was also evaluated. The cardioprotective effects were assessed in glucose-induced hypertensive rats after 21 days of treatment with AMECS (500 mg/kg single-daily oral dose). At the conclusion of the treatments, we investigated hemodynamic and biochemical parameters as well as endothelium-dependent vascular reactivity and tissue redox state. AMECS exhibited a significant antioxidant effect in vitro. In addition, no evidence of acute toxicity was observed. In glucose-induced hypertensive rats, AMECS prevented the increase in systolic blood pressure, oxidative stress, endothelial dysfunction, and metabolic alterations induced by glucose. The data obtained led us to conclude that the fruit extract of C. songarica presents a significant cardioprotective effect in glucose-induced hypertensive rats.
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