Although progress has been made to show the role of raw flaxseed and flaxseed extracts in health promotion, identification of mechanism(s) of action and molecule(s) underpinning beneficial effects largely remain unknown. The present study evaluated the efficacy of an aqueous flaxseed extract (AFE) to correct alloxan-induced diabetes in mice. Mice were divided into five groups: one nondiabetic (negative control) and four diabetic. Diabetic mice were treated with AFE, gallic acid (GA) (major component of AFE), insulin (positive control), or remained untreated (positive control). Oral administration of AFE strongly improved serum glucose, oral glucose tolerance, insulin tolerance, body weight, and polyphagia in diabetic mice. AFE was effective in controlling lipid peroxidation (thiobarbituric acid-reactive substances) and antioxidant enzymes (glutathione peroxidase, superoxide dismutase, and catalase) in liver and kidney, which undergo diabetes-related complications due to hyperglycemia. These results demonstrated that GA alone was sufficient to account for the beneficial health effects of AFE against diabetes.
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