Some lactic acid bacteria (LAB) and their cellular components have antiobesity effects. In this study, we evaluated the antiadipogenic effects of a mixture of two LAB—Lactobacillus curvatus HY7601 and Lactobacillus plantarum KY1032—using 3T3-L1 preadipocytes and HepG2 hepatocarcinoma cells. 3T3-L1 cells treated with a 1:1 ratio of HY7601 and KY1032 during differentiation showed reduced lipid accumulation by Oil Red O staining, as well as decreased leptin secretion and mRNA expression of peroxisome proliferator-activated receptor-γ and CCAAT/enhancer binding protein-α. HY7601 and KY1032 treatment also suppressed mitochondrial biogenesis and inhibited the expression of genes encoding mitochondrial transcription factors, as well as those related to fatty acid synthesis in HepG2 cells. The antiadipogenic effects of LAB were associated with the cell membrane fraction. These results demonstrate that a mixture of two LAB (HY7601 and KY1032) inhibits adipogenesis in preadipocytes and liver cells and is a potential therapeutic strategy for the treatment of obesity.
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References
1.
KopelmanPG: Obesity as a medical problem. Nature, 2000; 404:635–643.
2.
JeongYS, HongJH, ChoKH, JungHK: Grape skin extract reduces adipogenesis- and lipogenesis-related gene expression in 3T3-L1 adipocytes through the peroxisome proliferatoractivated receptor-gamma signaling pathway. Nutr Res, 2012; 32:514–521.
3.
WangS, Moustaid-MoussaN, ChenL, et al.: Novel insights of dietary polyphenols and obesity. J Nutr Biochem, 2014; 25:1–18.
4.
WuSJ, HuangGY, NgLT: Gamma-Tocotrienol induced cell cycle arrest and apoptosis via activating the Bax-mediated mitochondrial and AMPK signaling pathways in 3T3-L1 adipocytes. Food Chem Toxicol, 2013; 59:501–513.
5.
FlintHJ, ScottKP, LouisP, DuncanSH: The role of the gut microbiota in nutrition and health. Nat Rev Gastro Hepat, 2012; 9:577–589.
6.
BackhedF, DingH, WangT, et al.: The gut microbiota as an environmental factor that regulates fat storage. P Natl Acad Sci USA, 2004; 101:15718–15723.
7.
TurnbaughPJ, LeyRE, MahowaldMA, MagriniV, MardisER, GordonJI: An obesity-associated gut microbiome with increased capacity for energy harvest. Nature, 2006; 444:1027–1031.
8.
DiBaiseJK, ZhangH, CrowellMD, Krajmalnik-BrownR, DeckerGA, RittmannBE: Gut microbiota and its possible relationship with obesity. Mayo Clin Proc, 2008; 83:460–469.
9.
DahiyaDK, Renuka, PuniyaM, et al.: Gut microbiota modulation and its relationship with obesity using prebiotic fibers and probiotics: A review. Front Microbiol, 2017; 8:563.
10.
CaniPD, BibiloniR, KnaufC, et al.: Changes in gut microbiota control metabolic endotoxemia-induced inflammation in high-fat diet-induced obesity and diabetes in mice. Diabetes, 2008; 57:1470–1481.
11.
ParkS, BaeJH: Probiotics for weight loss: A systematic review and meta-analysis. Nutr Res, 2015; 35:566–575.
12.
AhnHY, KimM, AhnYT, et al.: The triglyceride-lowering effect of supplementation with dual probiotic strains, Lactobacillus curvatus HY7601 and Lactobacillus plantarum KY1032: Reduction of fasting plasma lysophosphatidylcholines in nondiabetic and hypertriglyceridemic subjects. Nutr Metab Cardiovas, 2015; 25:724–733.
13.
AhnHY, KimM, ChaeJS, et al.: Supplementation with two probiotic strains, Lactobacillus curvatus HY7601 and Lactobacillus plantarum KY1032, reduces fasting triglycerides and enhances apolipoprotein A-V levels in non-diabetic subjects with hypertriglyceridemia. Atherosclerosis, 2015; 241:649–656.
14.
ParkDY, AhnYT, ParkSH, et al.: Supplementation of Lactobacillus curvatus HY7601 and Lactobacillus plantarum KY1032 in diet-induced obese mice is associated with gut microbial changes and reduction in obesity. PLoS One, 2013; 8:e59470.
15.
YooSR, KimYJ, ParkDY, et al.: Probiotics L. plantarum and L. curvatus in combination alter hepatic lipid metabolism and suppress diet-induced obesity. Obesity, 2013; 21:2571–2578.
16.
WooM, KimM, NohJS, ParkCH, SongYO: Kimchi attenuates fatty streak formation in the aorta of low-density lipoprotein receptor knockout mice via inhibition of endoplasmic reticulum stress and apoptosis. Nutr Res Prac, 2017; 11:445–451.
17.
HanK, BoseS, WangJH, et al.: Contrasting effects of fresh and fermented kimchi consumption on gut microbiota composition and gene expression related to metabolic syndrome in obese Korean women. Mol Nutr Food Res, 2015; 59:1004–1008.
18.
KimEK, AnSY, LeeMS, et al.: Fermented kimchi reduces body weight and improves metabolic parameters in overweight and obese patients. Nutr Res, 2011; 31:436–443.
19.
ParkJE, OhSH, ChaYS: Lactobacillus brevis OPK-3 isolated from kimchi inhibits adipogenesis and exerts anti-inflammation in 3T3-L1 adipocyte. J Sci Food Agric, 2014; 94:2514–2520.
20.
OhYJ, JangJY, LimSK, et al.: Virgibacillus kimchii sp. nov., a halophilic bacterium isolated from kimchi. J Microbiol, 2017; 55:933–938.
21.
LeeK, LeeY: Effect of Lactobacillus plantarum as a starter on the food quality and microbiota of kimchi. Food Sci Biotechnol, 2010; 19:641–646.
22.
KimM, ChunJ: Bacterial community structure in kimchi, a Korean fermented vegetable food, as revealed by 16S rRNA gene analysis. Int J Food Microbiol, 2005; 103:91–96.
23.
MheenTI, KwonTW: Effect of temperature and salt concentration on kimchi fermentation. Korean J Food Sci Technol, 1984; 16:443–450.
24.
LeeSH, AhnMJ, HongJS, LeeJH: Diversity and community analysis of fermenting bacteria isolated from eight major Korean fermented foods using arbitrary-primed PCR and 16S rRNA gene sequencing. J Korean Soc Appl Biol Chem, 2015; 58:453–461.
25.
BleauC, LamontagneL, SavardR: New Lactobacillus acidophilus isolates reduce the release of leptin by murine adipocytes leading to lower interferon-gamma production. Clin Exp Immunol, 2005; 140:427–435.
26.
KimHG, GimMG, KimJY, et al.: Lipoteichoic acid from Lactobacillus plantarum elicits both the production of interleukin-23p19 and suppression of pathogen-mediated interleukin-10 in THP-1 cells. FEMS Immunol Med Microbiol, 2007; 49:205–214.
27.
YangJY, Della-FeraMA, BaileCA: Guggulsterone inhibits adipocyte differentiation and induces apoptosis in 3T3-L1 cells. Obesity, 2008; 16:16–22.
28.
ZangM, ZuccolloA, HouX, et al.: AMP-activated protein kinase is required for the lipid-lowering effect of metformin in insulin-resistant human HepG2 cells. J Biol Chem, 2004; 279:47898–47905.
29.
KaraskovE, ScottC, ZhangL, TeodoroT, RavazzolaM, VolchukA: Chronic palmitate but not oleate exposure induces endoplasmic reticulum stress, which may contribute to INS-1 pancreatic beta-cell apoptosis. Endocrinology, 2006; 147:3398–3407.
30.
SuryawanA, HuCY: Effect of serum on differentiation of porcine adipose stromal-vascular cells in primary culture. Comp Biochem Physiol Comp Physiol, 1993; 105:485–492.
31.
HouseknechtKL, BaileCA, MatteriRL, SpurlockME: The biology of leptin: A review. J Anim Sci, 1998; 76:1405–1420.
32.
ZeigererA, RodehefferMS, McGrawTE, FriedmanJM: Insulin regulates leptin secretion from 3T3-L1 adipocytes by a PI 3 kinase independent mechanism. Exp Cell Res, 2008; 314:2249–2256.
33.
BogackaI, XieH, BrayGA, SmithSR: Pioglitazone induces mitochondrial biogenesis in human subcutaneous adipose tissue in vivo. Diabetes, 2005; 54:1392–1399.
34.
HwangYP, ChoiJH, HanEH, et al.: Purple sweet potato anthocyanins attenuate hepatic lipid accumulation through activating adenosine monophosphate-activated protein kinase in human HepG2 cells and obese mice. Nutr Res, 2011; 31:896–906.
35.
MollerDE: New drug targets for type 2 diabetes and the metabolic syndrome. Nature, 2001; 414:821–827.
36.
ChoEJ, RahmanMA, KimSW, et al.: Chitosan oligosaccharides inhibit adipogenesis in 3T3-L1 adipocytes. J Microbiol Biotechnol, 2008; 18:80–87.
37.
MaffeiM, HalaasJ, RavussinE, et al.: Leptin levels in human and rodent: Measurement of plasma leptin and ob RNA in obese and weight-reduced subjects. Nat Med, 1995; 1:1155–1161.
38.
HavelPJ: Role of adipose tissue in body-weight regulation: Mechanisms regulating leptin production and energy balance. P Nutr Soc, 2000; 59:359–371.
39.
CristanchoAG, LazarMA: Forming functional fat: A growing understanding of adipocyte differentiation. Nat Rev Mol Cell Bio, 2011; 12:722–734.
40.
SmithPJ, WiseLS, BerkowitzR, WanC, RubinCS: Insulin-like growth factor-I is an essential regulator of the differentiation of 3T3-L1 adipocytes. J Biol Chem, 1988; 263:9402–9408.
41.
JeonT, HwangSG, HiraiS, et al.: Red yeast rice extracts suppress adipogenesis by down-regulating adipogenic transcription factors and gene expression in 3T3-L1 cells. Life Sci, 2004; 75:3195–3203.
42.
OberkoflerH, BeerA, BrebanD, HellE, KremplerF, PatschW: Human obese gene expression: Alternative splicing of mRNA and relation to adipose tissue localization. Obes Surg, 1997; 7:390–396.
43.
WuZ, XieY, BucherNL, FarmerSR: Conditional ectopic expression of C/EBP beta in NIH-3T3 cells induces PPAR gamma and stimulates adipogenesis. Genes Dev, 1995; 9:2350–2363.
44.
YangJ, RenJ, SongJ, et al.: Glucagon-like peptide 1 regulates adipogenesis in 3T3-L1 preadipocytes. Int J Mol Med, 2013; 31:1429–1435.
45.
GonzalesAM, OrlandoRA: Role of adipocyte-derived lipoprotein lipase in adipocyte hypertrophy. Nutr Metab, 2007; 4:22.
46.
LiKK, LiuCL, ShiuHT, et al.: Cocoa tea (Camellia ptilophylla) water extract inhibits adipocyte differentiation in mouse 3T3-L1 preadipocytes. Sci Rep, 2016; 6:20172.
47.
LinZ, ZhangB, LiuX, JinR, ZhuW: Effects of chicory inulin on serum metabolites of uric acid, lipids, glucose, and abdominal fat deposition in quails induced by purine-rich diets. J Med Food, 2014; 17:1214–1221.
48.
ZhangZ, ZhouZ, LiY, ZhouL, DingQ, XuL: Isolated exopolysaccharides from Lactobacillus rhamnosus GG alleviated adipogenesis mediated by TLR2 in mice. Sci Rep, 2016; 6:36083.
49.
LebeerS, BronPA, MarcoML, et al.: Identification of probiotic effector molecules: Present state and future perspectives. Curr Opin Botechnol, 2018; 49:217–223.
50.
LiS, BouzarC, Cottet-RousselleC, et al.: Resveratrol inhibits lipogenesis of 3T3-L1 and SGBS cells by inhibition of insulin signaling and mitochondrial mass increase. Biochim Biophys Acta, 2016; 1857:643–652.
51.
BogackaI, UkropcovaB, McNeilM, GimbleJM, SmithSR: Structural and functional consequences of mitochondrial biogenesis in human adipocytes in vitro. J Clin Endocr metab, 2005; 90:6650–6656.
52.
KaamanM, SparksLM, van HarmelenV, et al.: Strong association between mitochondrial DNA copy number and lipogenesis in human white adipose tissue. Diabetologia, 2007; 50:2526–2533.
53.
LiS, YaoY, XuR, et al.: Retraction for Li et al., “ATAD3 is a limiting factor in mitochondrial biogenesis and adipogenesis of white adipocyte-like 3T3-L1 cells.”. Mol Cellular Biol, 2017; 37:e01170-13.
54.
MuoioDM, SeefeldK, WittersLA, ColemanRA: AMP-activated kinase reciprocally regulates triacylglycerol synthesis and fatty acid oxidation in liver and muscle: Evidence that sn-glycerol-3-phosphate acyltransferase is a novel target. Biochem J, 1999; 338:783–791.
55.
SandersFW, GriffinJL: De novo lipogenesis in the liver in health and disease: More than just a shunting yard for glucose. Biol Rev Camb Philos Soc, 2016; 91:452–468.
56.
KuhajdaFP: Fatty-acid synthase and human cancer: New perspectives on its role in tumor biology. Nutrition, 2000; 16:202–208.
MoonYS, LatasaMJ, GriffinMJ, SulHS: Suppression of fatty acid synthase promoter by polyunsaturated fatty acids. J Lipid Res, 2002; 43:691–698.
59.
HortonJD, GoldsteinJL, BrownMS: SREBPs: Activators of the complete program of cholesterol and fatty acid synthesis in the liver. J Clin Invest, 2002; 109:1125–1131.
60.
YangJ, GoldsteinJL, HammerRE, MoonYA, BrownMS, HortonJD: Decreased lipid synthesis in livers of mice with disrupted Site-1 protease gene. P Natl Acad Sci USA, 2001; 98:13607–13612.
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