In this study, red ginseng extract (RGE) was converted into high-content minor ginsenosides by fermenting with Bgp1 enzymes at 37°C for 5 days. Compared to the RGE, the minor ginsenoside contents were increased in fermented red ginseng extract (FRGE). Moreover, the amount of minor ginsenosides such as Rh1 (11%) and Rg2 (16%) was slightly augmented, while the level of Rg3 (33%) was significantly increased after bioconversion. Furthermore, we also examined and compared the effect of RGE and FRGE on the differentiation and mineralization of preosteoblastic MC3T3-E1 cells. Similarly, the level of mRNA expression of intracellular alkaline phosphatase (ALP) activity, type-1 collagen (Col-I) was also increased. Based on the comparison, it is clear that the FRGE has improved effects on bone formation and differentiation of preosteoblastic MC3T3-E1 cells.
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References
1.
SenguptaS, TohSA, SellersLA, et al.: Modulating angiogenesis: The yin and the yang in ginseng. Circulation, 2004; 110:1219–1225.
YuanCS, WuJA, OsinskiJ: Ginsenoside variability in American ginseng samples. Am J Clin Nutr, 2002; 75:600–601.
4.
TawabMA, BahrU, KarasM, WurglicsM, Schubert-ZsilaveczM: Degradation of ginsenosides in humans after oral administration. Drug Metabol Dispos, 2003; 31:1065–1071.
5.
XuQF, FangXL, ChenDF: Pharmacokinetics and bioavailability of ginsenoside Rb1 and Rg1 from Panax notoginseng in rats. J Ethnopharmacol, 2003; 84:187–192.
6.
BaeEA, HanMJ, KimEJ, KimDH: Transformation of ginseng saponins to ginsenoside Rh2 by acids and human intestinal bacteria and biological activities of their transformants. Arch Pharm Res, 2004; 27:61–67.
7.
QuanLH, MinJW, JinY, WangC, KimYJ, YangDC: Enzymatic biotransformation of ginsenoside Rb1 to compound K by recombinant beta-glucosidase from Microbacterium esteraromaticum. J Agric Food Chem, 2012; 60:3776–3781.
8.
QuanLH, MinJW, SathiyamoorthyS, YangDU, KimYJ, YangDC: Biotransformation of ginsenosides Re and Rg1 into ginsenosides Rg2 and Rh1 by recombinant β-glucosidase. Biotechnol Lett, 2012; 34:913–917.
9.
PeiJ, PangQ, ZhaoL, FanS, ShiH: Thermoanaerobacterium thermosaccharolyticum β-glucosidase: A glucose-tolerant enzyme with high specific activity for cellobiose. Biotechnol Biofuels, 2012; 5:1–10.
10.
KoSR, ChoiKJ, SuzukiK, SuzukiY: Enzymatic preparation of ginsenosides Rg2, Rh1, and F1. Chem Pharm Bull, 2003; 51:404–408.
11.
BaeEA, ShinJE, KimDH: Metabolism of ginsenoside Re by human intestinal microflora and its estrogenic effect. Biol Pharm Bull, 2005; 28:1903–1908.
12.
ChoiJR, HongSW, KimY, et al.: Jang: Metabolic activities of ginseng and its constituents, ginsenoside Rb1 and Rg1, by human intestinal microflora. J Ginseng Res, 2011; 35:301–307.
13.
JiaL, ZhaoY: Current evaluation of the millennium phytomedicine-ginseng (I): Etymology, pharmacognosy, phytochemistry, market and regulations. Curr Med Chem, 2009; 16:2475–2484.
WattsNB: Bisphosphonate treatment of osteoporosis. Clin Geriatr Med, 2003; 19:395–414.
20.
QuarlesLD, YohayDA, LeverLW, CatonR, WenstrupRJ: Distinct proliferative and differentiated stages of murine MC3T3-E1 cells in culture: An in vitro model of osteoblast development. J Bone Miner Res, 1992; 7:683–690.
21.
LianJB, SteinGS, CanalisE, RobeyPG, BoskeyAL: Bone formation: Osteoblast lineage cells, growth factors, matrix proteins and the mineralization process. In: Primer on the Metabolic Bone Diseases and Disorders of Mineral Metabolism (FavusMJ, ed.). Lippincott Williams & Wilkins, Philadelphia, 1999, pp. 14–29.
22.
GreenLM, ReadeJL, WareCF: Rapid colorimetric assay for cell viability: Application to the quantitation of cytotoxic and growth inhibitory lymphokines. J Immunol Methods, 1984; 70:257–268.
23.
TietzNW, BurtisCA, DuncanP, ErvinK, PetitclercCJ, RinkerAD, ShueyD, ZygowiczER: A reference method for measurement of alkaline phosphatase activity in human serum. Clin Chem, 1983; 29:751–761.
24.
Tullberg-ReinertH, JundtG: In situ measurement of collagen synthesis by human bone cells with a sirius red-based colorimetric microassay: Effects of transforming growth factor beta2 and ascorbic acid 2-phosphate. Histochem Cell Biol, 1999; 112:271–276.
25.
VargheseJN, HrmovaM, FincherGB: Three-dimensional structure of a barley beta-D-glucan exohydrolase, a family 3 glycosyl hydrolase. Structure, 1999; 15:179–190.
26.
SykarasN, OppermanLA: Bone morphogenetic proteins (BMPs): How do they function and what can they offer the clinician?. J Oral Sci, 2003; 45:57–73.
27.
NoheA, KeatingE, KnausP, PetersenNO: Signal transduction of bone morphogenetic protein receptors. Cell Signal, 2004; 16:291–299.
28.
JiaTL, WangHZ, XieLP, WangXY, ZhangRQ: Daidzein enhances osteoblast growth that may be mediated by increased bone morphogenetic protein (BMP) production. Biochem Pharmacol, 2003; 65:709–715.
29.
KuoPL, HsuYL, ChangCH, ChangJK: Osthole-mediated cell differentiation through bone morphogenetic protein-2/p38 and extracellular signal-regulated kinase 1/2 pathway in human osteoblast cells. J Pharmacol Exp Ther, 2005; 314:1290–1299.
30.
KuoPL, HuangYT, ChangCH, ChangJK: Bone morphogenetic protein-2 and −4 (BMP-2 and −4) mediates fraxetin-induced maturation and differentiation in human osteoblast-like cell lines. Biol Pharm Bull, 2006; 29:119–124.
31.
GarrettIR: Anabolic agents and the bone morphogenetic protein pathway. Curr Top Dev Biol, 2007; 78:127–171.
32.
KimDY, ParkYG, QuanHY, KimSJ, JungMS, ChungSH: Ginsenoside Rd stimulates the differentiation and mineralization of osteoblastic MC3T3-E1 cells by activating AMP-activated protein kinase via the BMP-2 signaling pathway. Fitoterapia, 2012; 83:215–222.
33.
LeeMH, KimYJ, KimHJ, ParkHD, KangAR, KyungHM, SungJH, WozneyJM, RyooHM: BMP-2-induced Runx2 expression is mediated by Dlx5, and TGF-beta 1 opposes the BMP-2-induced osteoblast differentiation by suppression of Dlx5 expression. J Biol Chem, 2003; 278:34387–34394.
34.
GoriF, ThomasT, HicokKC, SpelsbergTC, RiggsBL: Differentiation of human marrow stromal precursor cells: Bone morphogenetic protein-2 increases OSF2/CBFA1, enhances osteoblast commitment, and inhibits late adipocyte maturation. J Bone Miner Res, 1999; 14:1522–1535
35.
PhimphilaiM, ZhoaZR, BoulesH, RocaH, FranceschiRT: BMP signaling is required for RUNX2-dependent induction of the osteoblast phenotype. J Bone Miner Res, 2006; 21:637–646.
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