We investigated the effect of carbohydrate ingestion after maximal lengthening contractions of the knee extensors on circulating concentrations of myocellular proteins and cytokines, and cytokine mRNA expression in muscle. Using a cross-over design, 10 healthy males completed 5 sets of 10 lengthening (eccentric) contractions (unilateral leg press) at 120% 1 repetition-maximum. Subjects were randomized to consume a carbohydrate drink (15% weight per volume; 3 g/kg BM) for 3 h after exercise using one leg, or a placebo drink after exercise using the contralateral leg on another day. Blood samples (10 mL) were collected before exercise and after 0, 30, 60, 90, 120, 150, and 180 min of recovery. Muscle biopsies (vastus lateralis) were collected before exercise and after 3 h of recovery. Following carbohydrate ingestion, serum concentrations of glucose (30–90 min and at 150 min) and insulin (30–180 min) increased (P < 0.05) above pre-exercise values. Serum myoglobin concentration increased (∼250%; P < 0.05) after both trials. In contrast, serum cytokine concentrations were unchanged throughout recovery in both trials. Muscle mRNA expression for IL-8 (6.4-fold), MCP-1 (4.7-fold), and IL-6 (7.3-fold) increased substantially after carbohydrate ingestion. TNF-α mRNA expression did not change after either trial. Carbohydrate ingestion during early recovery from exercise-induced muscle injury may promote proinflammatory reactions within skeletal muscle.
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References
1.
AljadaA, FriedmanJ, GhanimH, MohantyP, HofmeyerD, ChaudhuriA, DandonaP. 2006. Glucose ingestion induces an increase in intranuclear nuclear factor kappaB, a fall in cellular inhibitor kappaB, and an increase in tumor necrosis factor alpha messenger RNA by mononuclear cells in healthy human subjects. Metab Clin Exp, 55,9:1177–1185.
2.
ArkinstallMJ, TunstallRJ, Cameron-SmithD, HawleyJA. 2004. Regulation of metabolic genes in human skeletal muscle by short-term exercise and diet manipulation. Am J Physiol Endocrinol Metab, 287,1:E25–E31.
3.
ArnoldL, HenryA, PoronF, Baba-AmerY, van RooijenN, PlonquetA, GherardiRK, ChazaudB. 2007. Inflammatory monocytes recruited after skeletal muscle injury switch into antiinflammatory macrophages to support myogenesis. J Exp Med, 204,5:1057–1069.
4.
BeatonLJ, TarnopolskyMA, PhillipsSM. 2002. Contraction-induced muscle damage in humans following calcium channel blocker administration. J Physiol (Lond), 544,Pt 3:849–859.
5.
ChenYW, HubalMJ, HoffmanEP, ThompsonPD, ClarksonPM. 2003. Molecular responses of human muscle to eccentric exercise. J Appl Physiol, 95,6:2485–2494.
6.
CoffeyVG, PilegaardH, GarnhamAP, O'BrienBJ, HawleyJA. 2009. Consecutive bouts of diverse contractile activity alter acute responses in human skeletal muscle. J Appl Physiol, 106,4:1187–1197.
7.
DasuMR, DevarajS, JialalI. 2007. High glucose induces IL-1beta expression in human monocytes: mechanistic insights. Am J Physiol Endocrinol Metab, 293,1:E337–E346.
8.
DepnerCM, FredericksonSJ, RhodesK, BondK, BarryR, KirwanR, MilesMP. 2009. Post-exercise carbohydrates increase the magnitude of the inflammatory response. Seattle, WA: American College of Sports Medicine.
9.
DhindsaS, TripathyD, MohantyP, GhanimH, SyedT, AljadaA, DandonaP. 2004. Differential effects of glucose and alcohol on reactive oxygen species generation and intranuclear nuclear factor-kappaB in mononuclear cells. Metab Clin Exp, 53,3:330–334.
10.
DickinsonS, HancockDP, PetoczP, CerielloA, Brand-MillerJ. 2008. High-glycemic index carbohydrate increases nuclear factor-kappaB activation in mononuclear cells of young, lean healthy subjects. Am J Clin Nutr, 87,5:1188–1193.
11.
EvansWJ, PhinneySD, YoungVR. 1982. Suction applied to a muscle biopsy maximizes sample size. Med Sci Sports Exerc, 14,1:101–102.
HiroseL, NosakaK, NewtonM, LavederA, KanoM, PeakeJ, SuzukiK. 2004. Changes in inflammatory mediators following eccentric exercise of the elbow flexors. Exerc Immunol Rev, 10:75–90.
14.
HolmC, HörbrandF, MayrM, von DonnersmarckGH, MühlbauerW. 2004. Acute hyperglycaemia following thermal injury: friend or foe?Resuscitation, 60,1:71–77.
15.
HopkinsWG. 2006. Spreadsheets for analysis of controlled trials, with adjustment for a subject characteristic. Sportscience, 10:46–50.
16.
HopkinsWG, MarshallSW, BatterhamAM, HaninJ. 2009. Progressive statistics for studies in sports medicine and exercise science. Med Sci Sports Exerc, 41,1:3–13.
17.
HubalMJ, ChenTC, ThompsonPD, ClarksonPM. 2008. Inflammatory gene changes associated with the repeated-bout effect. Am J Physiol Regul Integr Comp Physiol, 294,5:R1628–R1637.
18.
LouisE, RaueU, YangY, JemioloB, TrappeS. 2007. Time course of proteolytic, cytokine, and myostatin gene expression after acute exercise in human skeletal muscle. J Appl Physiol, 103,5:1744–1751.
19.
MacIntyreDL, SorichterS, MairJ, BergA, McKenzieDC. 2001. Markers of inflammation and myofibrillar proteins following eccentric exercise in humans. Eur J Appl Physiol, 84,3:180–186.
20.
MilesMP, PearsonSD, AndringJM, KiddJR, VolpeSL. 2007. Effect of carbohydrate intake during recovery from eccentric exercise on interleukin-6 and muscle-damage markers. Int J Sport Nutr Exerc Metab, 17,6:507–520.
MowlaviA, AndrewsK, MilnerS, HerndonDN, HeggersJP. 2000. The effects of hyperglycemia on skin graft survival in the burn patient. Ann Plast Surg, 45,6:629–632.
23.
NiemanDC, BishopNC. 2006. Nutritional strategies to counter stress to the immune system in athletes, with special reference to football. J Sports Sci, 24,7:763–772.
24.
NiemanDC, DavisJM, BrownVA, HensonDA, DumkeCL, UtterAC, VinciDM, DownsMF, SmithJC, CarsonJ, BrownA, McAnultySR, McAnultyLS. 2004. Influence of carbohydrate ingestion on immune changes after 2 h of intensive resistance training. J Appl Physiol, 96,4:1292–1298.
25.
NiemanDC, DavisJM, HensonDA, GrossSJ, DumkeCL, UtterAC, VinciDM, CarsonJA, BrownA, McAnultySR, McAnultyLS, TriplettNT. 2005. Muscle cytokine mRNA changes after 2.5 h of cycling: influence of carbohydrate. Med Sci Sports Exerc, 37,8:1283–1290.
NiemanDC, HensonDA, DavisJM, DumkeCL, GrossSJ, JenkinsDP, MurphyEA, CarmichaelMD, QuindryJC, McAnultySR, McAnultyLS, UtterAC, MayerEP. 2007. Quercetin ingestion does not alter cytokine changes in athletes competing in the Western States Endurance Run. J Interferon Cytokine Res, 27,12:1003–1011.
28.
NiemanDC, HensonDA, SmithLL, UtterAC, VinciDM, DavisJM, KaminskyDE, ShuteM. 2001. Cytokine changes after a marathon race. J Appl Physiol, 91,1:109–114.
29.
NiemanDC, Nehlsen-CannarellaSL, FagoagaOR, HensonDA, UtterA, DavisJM, WilliamsF, ButterworthDE. 1998. Influence of mode and carbohydrate on the cytokine response to heavy exertion. Med Sci Sports Exerc, 30,5:671–678.
30.
NosakaK, SaccoP, MawatariK. 2006. Effects of amino acid supplementation on muscle soreness and damage. Int J Sport Nutr Exerc Metab, 16,6:620–635.
31.
PaulsenG, BenestadHB, Strøm-GundersenI, MørkridL, LappegårdKT, RaastadT. 2005. Delayed leukocytosis and cytokine response to high-force eccentric exercise. Med Sci Sports Exerc, 37,11:1877–1883.
32.
PeakeJ, NosakaK, SuzukiK. 2005. Characterization of inflammatory responses to eccentric exercise in humans. Exerc Immunol Rev, 11:64–85.
33.
PeakeJM, NosakaK, MuthalibM, SuzukiK. 2006. Systemic inflammatory responses to maximal versus submaximal lengthening contractions of the elbow flexors. Exerc Immunol Rev, 12:72–85.
34.
PhillipsT, ChildsAC, DreonDM, PhinneyS, LeeuwenburghC. 2003. A dietary supplement attenuates IL-6 and CRP after eccentric exercise in untrained males. Med Sci Sports Exerc, 35,12:2032–2037.
35.
RaastadT, RisoyBA, BenestadHB, FjeldJG, HallenJ. 2003. Temporal relation between leukocyte accumulation in muscles and halted recovery 10–20 h after strength exercise. J Appl Physiol, 95,6:2503–2509.
36.
SekineO, NishioY, EgawaK, NakamuraT, MaegawaH, KashiwagiA. 2002. Insulin activates CCAAT/enhancer binding proteins and proinflammatory gene expression through the phosphatidylinositol 3-kinase pathway in vascular smooth muscle cells. J Biol Chem, 277,39:36631–36639.
