"Discordant" Influence of Equine Recombinant Interferon-β1 on the Cytotoxic Capacity of Equine Polymorphonuclear Neutrophils and Peripheral Blood Mononuclear Cells In Vitro and In Vivo

Abstract

The influence of recombinant equine interferon-β₁ (rEqIFN-β₁) on mononuclear cells of peripheral blood (PBMC) and polymorphonuclear neutrophilic granulocytes (PMN) was tested under in vitro and ex vivo conditions. Treatment of equine PBMC with IFN in vitro enhanced the antibody-independent cytotoxicity (AICC) and antibody-dependent cytotoxicity (ADCC) while there was no significant effect on the cytotoxic capacity of PMN treated with rEqIFN-β₁ in vitro. Ex vivo there was an increased capacity of AICC and ADCC upon single or multiple application of rEqIFN-β₁ in PMN, only. Treatment with rEqIFN-β₁ thus induced an increased cellular cytotoxicity in vitro and in vivo but in different populations of peripheral blood cells. In vivo rEqIFN-β₁ causes a pronounced activation of PMN but not of PBMC as cytotoxic effector cells. This might be achieved indirectly, e.g., by cytokines produced by IFN-sensitive cells.