Abstract
Most studies on the immunomodulating effects of interferon-γ IFN-γ) have been performed in vitro, using recombinant mouse and human IFN-γ preparations. Recently, recombinant rat IFN-γ (rRIFN-γ) became available, enabling extensive studies with this new preparation in vivo. In the present study a LEW rat model was used to determine the efficacy of rRIFN-γ on immune functions in vivo. LEW rats were treated with rRIFN-γ by continuous intravenous infusion at a dosage of 1.5 × 105 U/kg· h for 2 consecutive days. Twelve hours after cessation of rRIFN-γ administration immune functions, including NK-cell activity, phagocytosis, and mitogen-induced blastogenesis, were assessed. All experimental animals displayed a marked reduction in the number of peripheral blood and bone marrow cells when compared with controls (p < 0.005). Assessment of immune functions revealed a significant enhancement of NK-cell activity (p < 0.001), phagocytosis (p < 0.05), and mitogen-induced blastogenesis (p < 0.05). These findings indicate that rRIFN-γ, when given in high dosages, has a stimulatory effect on various immune functions, which substantiates its important immunological role in vivo.
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