Abstract
Murine peritoneal macrophages were treated with interferon-γ (IFN-γ) or tumor necrosis factor-α (TNF). Measurements of changes in acid phosphatase and β-glucuronidase levels were made as an indication of activation by cytokine treatment. IFN-γ or TNF-γ treatment resulted in a significant increase in the activities of both enzymes measured in the cell lysates. This increase was observable after 6 h of incubation, but reached its maximum level after 24 h of incubation. The effect of the treatment of the cells with both cytokines together was additive. No synergistic effect of addition of both cytokines on the enzyme levels was observed.
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