Effect of Indomethacin,Aspirin,and Acetaminophen on In Vitro Antiviral and Antiproliferative Activities of Recombinant Human Interferon-α 2a

Abstract

The effects of indomethacin, aspirin, and acetaminophen on the antiviral and antiproliferative activities of recombinant human interferon-α_2a (rIFN-α_2a) were studied in vitro. None of the drugs inhibited the antiviral activity of rIFN-α_2a in human amnion FL cells against vesicular stomatitis virus, or interfered with its antiproliferative activity against acute lymphoblastic leukemia MOLT-4 cells or renal cell carcinoma NC 65 cells. Although, at high concentrations, aspirin (1 mM) or indomethacin (0.1 mM) alone inhibited the cell growth, rIFN-α_2a showed clear additive growth inhibition. It was concluded that neither indomethacin, aspirin, nor acetaminophen directly inhibited the antiviral and antiproliferative activities of rIFN-α_2a. The possible use of these three drugs to reduce the adverse effects of rIFN-α_2a without spoiling its profitable efficacy in clinical practice is suggested.