Abstract
The in vivo regulation of natural killer (NK) cell function by recombinant murine gamma interferon (rMuIFN-γ) and recombinant human interleukin-2 (rHuIL-2) was investigated. Peritoneal exudate-derived natural killer (PE-NK) cells of mice treated with rMuIFN-γ or rHuIL-2 exhibited significantly enhanced cytolytic activities against YAC-1 target cells. Compared with animals receiving treatment with either rMuIFN-γ or rHuIL-2 alone, the sequential treatment of mice with both agents resulted in a significantly greater enhancement of PE-NK cell function, especially when rMuIFN-γ was administered 24 h before treatment with rHuIL-2. The cytolytic activities were significantly diminished after pre-treatment of effector cells with anti-Qa-5 antiserum and complement but not with anti-Lyt-3.2 antiserum. These data constitute the first evidence demonstrating the association between IFN-γ and IL-2 in the regulation of NK cell function in vivo, reinforce the potential benefit of combined treatment with biological response modifiers, and show that the schedule of administration may be a critical requirement for optimal benefits of combined lymphokine treatment in vivo.
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