Detection of Hybridomas Secreting Monoclonal Antibodies to Human Gamma Interferon Using a Rapid Screening Technique and Specificity of Certain Monoclonal Antibodies to Gamma Interferon

Abstract

An immunoprecipitation technique, which uses radiolabeled human gamma interferon (HuIFN-γ) and a sheep anti-mouse F(ab')₂ serum, is described for the rapid detection of mouse monoclonal antibodies to IFN-γ in hybridoma culture supernatants. This technique which can be performed in 4 h detects both neutralizing and nonneutralizing antibodies to IFN-γ. Certain monoclonal antibodies prepared against natural (glycosylated) IFN-γ which neutralized the antiviral activity of natural IFN-γ bound strongly to rDNA (unglycosylated) IFN-γ derived from E. coli, but failed to neutralize its antiviral activity.