Abstract
Various mouse IFNs induced by poly(I) · poly(C) or poly(ICLC) were analyzed for the antigenic types by neutralization tests using anti-IFN-α and anti-IFN-β antibodies. The IFN samples included IFNs produced by L cells, by spleen cells in vitro and ex vivo, and in plasma of mice injected with the inducers. L-cell IFN was estimated to consist of IFN-β and IFN-β at a ratio of about 80:20. Spleen IFNs also consisted of both IFN species, but IFN-α was the major component. Blood IFN samples obtained 1-4 h after injection of poly(I) · poly(C) or poly(ICLC) consisted of the two molecular species, whereas the samples 8 and 24 h after injection contained only IFN-α. High levels of IFN activity (IFN-α) persisted much longer in the circulation of mice injected with poly (ICLC) than in mice injected with poly(I) · poly(C).
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