Abstract
A monoclonal antibody against murine interferon-beta (MuIFN-β) was prepared using standard methods. Antibodies were immobilized by coupling to Sepharose and used for large-scale purification of poly(I) · poly(C)-induced mouse L cell IFN. Antibodies isolated from the serum of one nude mouse which was transplanted with the anti-MuIFN-β antibodies producing hybridoma were able to bind at least 7 × 107U MuIFN-β.
In one single antibody affinity chromatography step MuIFN-α was separated from MuIFN-β and a 1000-fold purification of MuIFN-β was obtained. The purified material had a specific activity of 5 × 108U/mg protein. The recovery from the antibody column was 100%. SDS-PAGE analysis of the purified material revealed the presence of one single protein band with a molecular weight of 33 kD, representing MuIFN-β.
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