Abstract
The treatment of cultures of human splenic lymphocytes with Staphylococcal enterotoxin A (SEA) resulted in the production of human gamma interferon (HuIFN-γ). Separation of lymphocyte populations to give preparations enriched in T or B lymphocytes after removal of macrophages demonstrated that maximum IFN titers were detected when T lymphocytes were incubated with macrophages for a period of three days. The levels of IFN-γ varied from donor to donor but the kinetics of induction were quite similar in all cases. Concentrations of 0.5-1.0 μg/ml SEA gave optimal induction of IFN and the addition of thymosin α-1 at 10 μg/ml to the culture medium enhanced IFN-γ production.
Get full access to this article
View all access options for this article.