Purification of Human Lymphoblastoid Cell-Derived Interferon-Alpha by Controlled-Pore Glass Bead Adsorption Chromatography and Molecular Sieving*

Abstract

Human lymphoblastoid cell-derived interferon-alpha has been purified by a combination of chromatography on Controlled-Pore Glass 350, gel filtration on Ultrogel AcA 54 and hydrophobic chromatography on Phenyl-Sepharose CL-4B. Specific activities of > 10⁷ units/mg have been obtained. Controlled-Pore Glass has the advantages of accommodating large volumes of induced medium in an "in-line" process giving high recoveries of interferon and removing most of any contaminating DNA.