Osteoporosis is defined as a bone mass reduction, reaching a point that is insufficient to maintain the structural integrity of the skeleton, particularly after menopause. Caffeine (CAF) is associated with increased risk of osteoporotic bone fractures and inhibition of osteoblasts proliferation.
Objective:
The aim of this study was to produce an experimental menopause in ovariectomized (OVX) rats treated with daily caffeine injection and assess the newly formed bone.
Methods and Materials:
Sixty days after ovariectomy, 84 rats were subjected to extraction of upper right incisor and divided into sham: daily intraperitoneal injection of saline; CAF: daily injection of 1.5% caffeine (0.2 mL/100 g body weight) by the same period; OVX: ovariectomized and daily injection of saline; and OVX+CAF: ovariectomized and daily injection of 1.5% caffeine (0.2 mL/100 g body weight). The animals were killed on 7th, 14th, and 28th days after surgery, and their maxillae were processed for obtaining semiserial sections (5 μm) stained with hematoxylin and eosin. Through an image analysis system, the bone volume and the quality of newly formed bone in the dental socket were evaluated.
Results:
The histological results showed immature bone trabeculae and large amount of blood clot, and histometric analysis revealed less amount of new bone formation by 40.0% in osteoporotic animals and 61.0% in osteoporotic animals treated with caffeine compared with controls (ANOVA p≤0.01).
Conclusions:
It was concluded that castration/osteoporosis caused a delay in the repair of bone defects, and this delay was exacerbated by caffeine.
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