Restricted accessResearch articleFirst published online 2025-02
Characterization of Partially Purified Manganese Peroxidase from Soil-Derived Aspergillus Species and Its Biodegradation Potential Against Anthraquinone-Based Dye
The industrial effluent generates various environmental issues as it contains synthetic dyestuffs and chemicals. In the present study, the decolorization ability of soil-derived fungal isolates Aspergillus terreus and Aspergillus fumigatus was determined against anthraquinone-based Remazol Brilliant Blue Reactive (RBBR) dye. The dye degradation was characterized using UV-spectrophotometry, high-performance liquid chromatography, and gas chromatography-mass spectroscopy techniques. Moreover, manganese peroxidase (MnP) was partially purified for decolorization activity from fungal isolates. RBBR dye was 84.9% decolorized by A. terreus, while A. fumigatus decolorized 78.6% dye. The maximum decolorization activity of partially purified free and immobilized MnP enzyme from A. terreus was 86% and 93.7%, followed by A. fumigatus 80.3% and 89.7%, respectively. The molecular weight of A. fumigatus and A. terreus MnP enzyme were 43 and 45 kDa by sodium dodecyl sulfate–polyacrylamide gel electrophoresis. The scanning electron microscopy (SEM) images showed that the immobilization of MnP was found to be better resistant to optimum pH and temperature. The immobilized and free MnP enzyme decolorization activity from A. fumigatus was 89.7% and 80.3%, while from A. terreus had 93.7% and 86.1%, respectively. In conclusion, soil fungi can decolorize and degrade anthraquinone-based dyes such as RBBR. Moreover, the fungal MnP enzyme can be used to eradicate the toxic effect of industrial effluents.
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References
1.
Es-SahbanyH, BerradiM, NkhiliS, et al.Removal of heavy metals (nickel) contained in wastewater-models by the adsorption technique on natural clay. Materials Today: Proceedings, 2019; 13:866–875.
2.
BerradiM, HsissouR, KhudhairM, et al.Textile finishing dyes and their impact on aquatic environs. Heliyon, 2019; 5(11):e02711.
3.
PierceJ. Colour in textile effluents‐the origins of the problem. J Soc Dyers and Colourists, 1994; 110(4):131–133.
4.
VandeviverePC, BianchiR, VerstraeteW. Treatment and reuse of wastewater from the textile wet‐processing industry: Review of emerging technologies. J Chem Technol Biotechnol, 1998; 72(4):289–302.
5.
BeydilliMI, PavlostathisSG, TincherWC. Biological decolorization of the azo dye reactive red 2 under various oxidation–reduction conditions. Water Environ Res, 2000; 72(6):698–705.
6.
PearceC, LloydJ, GuthrieJ. The removal of colour from textile wastewater using whole bacterial cells: A review. Dyes and Pigments, 2003; 58(3):179–196.
7.
SlokarYM, Le MarechalAM. Methods of decoloration of textile wastewaters. Dyes and Pigments, 1998; 37(4):335–356.
8.
WeberEJ. Reactive dyes in the aquatic environment: A case study of Reactive Blue 19. US Environmental Protection Agency, Environmental Research Laboratory, 1990.
9.
HaoOJ, KimH, ChiangP-C. Decolorization of wastewater. Critical Reviews in Environ Sci Techno, 2000; 30(4):449–505.
10.
BanatIM, NigamP, SinghD, et al.Microbial decolorization of textile-dyecontaining effluents: A review. Bioresource Technol, 1996; 58(3):217–227.
11.
O’NeillC, HawkesFR, HawkesDL, et al.Colour in textile effluents–sources, measurement, discharge consents and simulation: A review. J Chem Technol Biotechnol, 1999; 74(11):1009–1018.
12.
ImranM, CrowleyDE, KhalidA, et al.Microbial biotechnology for decolorization of textile wastewaters. Rev Environ Sci Biotechnol, 2015; 14(1):73–92.
13.
VarjaniS, RakholiyaP, NgHY, et al.Microbial degradation of dyes: An overview. Bioresour Technol, 2020; 314:123728.
14.
RehmanK, ShahzadT, SaharA, et al.Effect of Reactive Black 5 azo dye on soil processes related to C and N cycling. PeerJ, 2018; 6:e4802.
PaździorK, WrębiakJ, Klepacz-SmółkaA, et al.Influence of ozonation and biodegradation on toxicity of industrial textile wastewater. J Environ Manage, 2017; 195(Pt 2):166–173.
17.
LinS, WeiJ, YangB, et al.Bioremediation of organic pollutants by white rot fungal cytochrome P450: The role and mechanism of CYP450 in biodegradation. Chemosphere, 2022; 301:134776.
18.
ChenS, ZhuM, GuoX, et al.Coupling of Fenton reaction and white rot fungi for the degradation of organic pollutants. Ecotoxicol Environ Saf, 2023; 254:114697.
19.
GaoX, WeiM, ZhangX, et al.Copper removal from aqueous solutions by white rot fungus Pleurotus ostreatus GEMB-PO1 and its potential in co-remediation of copper and organic pollutants. Bioresour Technol, 2024; 395:130337.
20.
ChenL, ZhangX, ZhangM, et al.Removal of heavy-metal pollutants by white rot fungi: Mechanisms, achievements, and perspectives. JCleaner Product, 2022; 354:131681.
21.
HadibarataT, AdnanLA, YusoffARM, et al.Microbial decolorization of an azo dye reactive black 5 using white-rot fungus Pleurotus eryngii F032. Water Air Soil Pollut, 2013; 224(6):1–9.
22.
MousaviSM, HashemiSA, Iman MoezziSM, et al.Recent advances in enzymes for the bioremediation of pollutants. Biochem Res Int, 2021; 2021(1):5599204.
23.
QinX, SunX, HuangH, et al.Oxidation of a non-phenolic lignin model compound by two Irpex lacteus manganese peroxidases: Evidence for implication of carboxylate and radicals. Biotechnol Biofuels, 2017; 10:103–113.
24.
XuH, GuoM-Y, GaoY-H, et al.Expression and characteristics of manganese peroxidase from Ganoderma lucidum in Pichia pastoris and its application in the degradation of four dyes and phenol. BMC Biotechnol, 2017; 17(1):19.
25.
Martı’nezAT. Molecular biology and structure-function of lignin-degrading heme peroxidases. Enzyme and Microbial Technology, 2002; 30(4):425–444.
26.
ChowdharyP, RajA, BharagavaRN. Environmental pollution and health hazards from distillery wastewater and treatment approaches to combat the environmental threats: A review. Chemosphere, 2018; 194:229–246.
27.
NovotnýČ, SvobodováK, ErbanováP, et al.Ligninolytic fungi in bioremediation: Extracellular enzyme production and degradation rate. Soil Biology and Biochemistry, 2004; 36(10):1545–1551.
28.
RohiniA, RajaVU, RajaA. Bioremediation of azo dyes by isolated fungal strains adopted from textile effluent-biosorption and biodegradation study. 2019.
29.
IlyasS, RehmanA. Decolorization and detoxification of Synozol red HF-6BN azo dye, by Aspergillus niger and Nigrospora sp. Iranian J Environ Health Sci Eng, 2013; 10(1):12–19.
30.
KhanSA, MehmoodS, IqbalA, et al.Industrial polluted soil borne fungi decolorize the recalcitrant azo dyes Synozol red HF–6BN and Synozol black B. Ecotoxicol Environ Saf, 2020; 206:111381.
31.
ZhaoX, HardinIR, HwangH-M. Biodegradation of a model azo disperse dye by the white rot fungus Pleurotus ostreatus. International Biodeterioration & Biodegradation, 2006; 57(1):1–6.
32.
AhmedZ. Enzymatic and Bacterial Modifications of Natural Biofibres for Synthesis of Biocomposites. University of Agriculture: Faisalabad; 2016.
33.
UrzúaU, KerstenPJ, VicuñaR. Manganese peroxidase-dependent oxidation of glyoxylic and oxalic acids synthesized by Ceriporiopsis subvermispora produces extracellular hydrogen peroxide. Appl Environ Microbiol, 1998; 64(1):68–73.
34.
MontiagueE, JpmdG, UncianoN, et al.Biodecolourization of Textile Dye and Wastewaters by Crude Laccase from Pleurotus florida ITDI 6003 Cultivated in Wheat Grains. CREAM, 2020; 10(1):167–175.
35.
BradfordMM. A rapid and sensitive method for the quantitation of microgram quantities of protein utilizing the principle of protein-dye binding. Anal Biochem, 1976; 72(1–2):248–254.
36.
RehmanHU, AmanA, ZohraRR, et al.Immobilization of pectin degrading enzyme from Bacillus licheniformis KIBGE IB-21 using agar-agar as a support. Carbohydr Polym, 2014; 102:622–626.
37.
Muhammad Nasir IqbalH, AsgherM. Characterization and decolorization applicability of xerogel matrix immobilized manganese peroxidase produced from Trametes versicolor IBL-04. Protein Pept Lett, 2013; 20(5):591–600.
38.
LouX, ZhaoJ, LouX, et al.The biodegradation of soil organic matter in soil-dwelling humivorous fauna. Front Bioeng Biotechnol, 2021; 9:808075.
39.
JathannaNN, KrishnamurthyGK, GowdruMS, et al.Deciphering phyto-fungal dye concoction mitigation, degraded metabolite analysis, and assessment of ecotoxicity. J Environ Chem Eng, 2024; 12(2):112171.
40.
KhalidA, BatoolS, SiddiqueMT, et al.Decolorization of Remazol Black-B azo dye in soil by fungi. Soil Environ, 2011; 30(1):1–6.
41.
AmeenF, AlshehreiF. Biodegradation optimization and metabolite elucidation of Reactive Red 120 by four different Aspergillus species isolated from soil contaminated with industrial effluent. Ann Microbiol, 2017; 67(4):303–312.
42.
IscenC, GülÜ, YavuzA, et al.Decolorization of dye solution containing Remazol Black B by Aspergillus niger isolated from hypersaline environment. Int J Environ Sci Technol, 2022; 19(12):12497–12504.
43.
YantoD, AulianaN, AnitaS, et al.Decolorization of Synthetic Textile Dyes by Laccase from Newly Isolated Trametes Hirsuta EDN084 Mediated by Violuric Acid. IOP Publishing: 2019.
44.
YantoDHY, AnitaSH, SolihatNN. Enzymatic degradation and metabolic pathway of acid blue 129 dye by crude laccase from newly isolated Trametes hirsuta EDN 082. Biocatalysis and Biotransformation, 2024; 42(2):129–139.
45.
PatelAM, PatelVM, PandyaJ, et al.Evaluation of catalytic efficiency of Coriolopsis caperata DN laccase to decolorize and detoxify RBBR dye. Water Conserv Sci Eng, 2017; 2(3):85–98.
46.
PerlattiB, da SilvaMFdGF, FernandesJB, et al.Validation and application of HPLC–ESI-MS/MS method for the quantification of RBBR decolorization, a model for highly toxic molecules, using several fungi strains. Bioresour Technol, 2012; 124:37–44.
47.
RoutoulaE, PatwardhanSV. Degradation of anthraquinone dyes from effluents: A review focusing on enzymatic dye degradation with industrial potential. Environ Sci Technol, 2020; 54(2):647–664.
48.
RogalskiJ, SzczodrakJ, JanuszG. Manganese peroxidase production in submerged cultures by free and immobilized mycelia of Nematoloma frowardii. Bioresour Technol, 2006; 97(3):469–476.
49.
HomaeiAA, SaririR, VianelloF, et al.Enzyme immobilization: An update. J Chem Biol, 2013; 6(4):185–205.
50.
LueangjaroenkitP, TeerapatsakulC, SakkaK, et al.Two manganese peroxidases and a laccase of Trametes polyzona KU-RNW027 with novel properties for dye and pharmaceutical product degradation in redox mediator-free system. Mycobiology, 2019; 47(2):217–229.
51.
BilalM, AsgherM, ShahidM, et al.Characteristic features and dye degrading capability of agar-agar gel immobilized manganese peroxidase. Int J Biol Macromol, 2016; 86:728–740.
52.
dos Santos BazanellaGC, AraujoA, CastoldiR, et al.Ligninolytic Enzymes from White-Rot Fungi and Application in the Removal of Synthetic Dyes. Fungal enzymes CRC Press, Boca Raton. 2013; 258–279.
53.
MesbahNM, WiegelJ. Improvement of activity and thermostability of agar-entrapped, thermophilic, haloalkaliphilic amylase AmyD8. Catal Lett, 2018; 148(9):2665–2674.
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