Butanol is an important bulk chemical and considered an advanced biofuel with better properties than ethanol. It can be produced by acetone–butanol–ethanol (ABE) or isopropanol-butanol-ethanol (IBE) fermentation by various Clostridium spp. In this work, eucalyptus wood pretreated in a steam explosion continuous type reactor and enzymatically hydrolyzed was used as culture medium. The effect of corn steep liquor (CSL), a low-cost source of protein, vitamins and minerals, to the enzymatic hydrolysate as a substitute for both P2 stock vitamin solution and yeast extract on ABE and IBE fermentation performance was studied. C. beijerinckii DSM 6422 and C. beijerinckii DSM 6423 were used for ABE and IBE production, respectively. No significant difference was observed for butanol concentration, yield, and productivity when CSL was used instead of P2 stock vitamin solution in both ABE and IBE fermentations (p > 0.05). Therefore, CSL could replace P2 stock vitamin solution without significant detriment to the production of butanol by both ABE or IBE fermentation.
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