Nanotechnology presents one of the most promising solutions to developing and improving devices for detection and identification of semiochemicals. The present work reports the development of a novel nanostructured sensor with high sensitivity and limit of detection. Polyaniline (PANI) was used as a recognition layer for the trace level detection of methyl-2,6,10 trimethyltridecanoate sex pheromone. The sensitive layer was characterized by atomic force microscopy during each layer deposition and uniform films with nanoscale thickness (87 nm) and a granular morphology were observed. The nanostructured cantilever demonstrated good performance and ability to detect the semiochemical of brown stink bug, with wide linearity range and high detection limits of 1.44 fg/mL. Therefore, this work suggests a design for a highly sensitive cantilever-based nanosensor for the detection of the sex pheromone of the brown stink bug.
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References
1.
Blassioli-MoraesMC, LaumannRA, OliveiraMWM, et al.Sex pheromone communication in two sympatric neotropical stink bug species Chinavia ubica and Chinavia impicticornis. J Chem Ecol, 2012; 38(7):836–845.
MoraesMCB, ParejaM, LaumannRA, et al.Response of the parasitoid Telenomus podisi to induced volatiles from soybean damaged by stink bug herbivory and oviposition. J Plant Interact, 2008; 32(2):102–112.
5.
WeberDC, MorrisonWR, KhrimianA, et al.Chemical ecology of Halyomorpha halys: Discoveries and applications. J Pest Sci, 2017; 90(4)989–1008.
6.
SonenshineDE. Pheromones: Function and use in insect and tick control. Life Sci, 2017; 6(2):407–459.
SteffensC, ManzoliA, OliveiraJE, et al.Bio-inspired sensor for insect pheromone analysis based on polyaniline functionalized AFM cantilever sensor. Sensor Actuat B Chem, 2014; 191:643–649.
9.
ShoaibM, HishamN, BasheerN, TariqM. Frequency and displacement analysis of electrostatic cantilever-based MEMS sensor. Analog Integr Circuits Signal Process, 2016; 88(1):1–11.
10.
OlssonSB, HanssonBS. Electroantennogram and single sensillum recording in insect antennae. Pheromone Signaling, 2013; 1068:157–177.
11.
SakuraiT, NamikiS, KanzakiR. Molecular and neural mechanisms of sex pheromone reception and processing in the silkmoth Bombyx mori. Front Physiol, 2014; 5:1–20.
12.
RozemarieML, AndreiB, LilianaH, et al.Electrospun based polyaniline sensors—A review. IOP Conf Ser Mater Sci Eng, 2017; 209:1–10.
13.
SteffensC, ManzoliA, PaschoalinRT, et al.Tracing paper substrate used for development of interdigitated graphite electrode and its application as humidity sensor. Synth Met, 2013; 183:36–39.
14.
BaiH, ShiG. Gas sensors based on conducting polymers. Sensors, 2007; 7(3):267–307.
15.
RamMK, SalernoM, AdamiM, et al.Physical properties of polyaniline films: Assembled by the layer-by-layer technique. Langmuir, 1999; 13(13):1252–1259.
ManzoliA, ShimizuFM, MercanteLA, et al.Layer-by-layer fabrication of AgCl-PANI hybrid nanocomposite films for electronic tongues. Phys Chem Chem Phys, 2014; 16(44):24275–24281.
18.
SilvaJS, BarrosA, ConstantinoCJL, et al.Layer-by-layer films based on carbon nanotubes and polyaniline for detecting 2-chlorophenol. J Nanosci Nanotechnol, 2014; 14:6586–6592.
19.
ChenG, ThundatT, WachterEA, WarmackRJ. Adsorption-induced surface stress and is effects on resonance frequency of microcantilevers. J Appl Phys, 1995; 77(8):3618–3622.
20.
BorgesM, SchmidtFGV, SujjiER, et al.Field responses of stink bugs to the natural and synthetic pheromone of the neotropical brown stink bug, Euschistus heros (Heteroptera: Pentatomidae). Physiol Entomol, 1998; 23(3):202–207.
21.
DuXS, XiaoM, MengYZ. Synthesis and characterization of polyaniline/graphite. J Polym Sci, 2004; 42(8):1972–1978.
22.
WangJ, FengB, WuW, HuangY. Chemisorption sensing and analysis using silicon cantilever sensor based on n-type metal-oxide-semiconductor transistor. Microelectron Eng, 2011; 88:1019–1023.
23.
GalianiPD, MalmongeJA, SantosDP, MalmongeLF. Compósitos de borracha natural com polianilina. Polímeros, 2007; 17(2):93–97.
24.
OgawaT, KomatsuM. Analysis of Q-burst waveforms. Radio Sci, 2007; 42:1–11.
25.
LiL, FerngL, WeiY, et al.Effects of acidity on the size of polyaniline-poly(sodium 4-styrenesulfonate) composite particles and the stability of corresponding colloids in water. J Colloid Interface Sci, 2012; 381(1):11–16.
26.
GajesdranP, SaraswathiR. Polyaniline-carbon nanotube composites. Pure Appl Chem, 2008; 80(11):2377–2395.
27.
LiuS, ZhuK, ZhangY, XuJ. Cyclic polyaniline nanostructures from aqueous/organic interfacial polymerization induced by polyacrylic acid. Polymer, 2006; 47:7680–7683.
28.
ButoiB, GrozaA, DincaP, et al.Morphological and structural analysis of polyaniline and poly(o-anisidine) layers generated in a dc glow discharge plasma by using an oblique angle electrode deposition configuration. Polymers, 2017; 9(732):1–18.
29.
LeeH-Y, RweiS-P, WangL, ChenP-H. Preparation and characterization of core–shell polyaniline–polystyrene sulfonate@Fe3O4 nanoparticles. Mater ChemPhys, 2008; 112(3):805–809.
30.
FujimotoK, KimJH, OlmoriK, et al.Flexible multilayer electrode films consisted of polyaniline and polyeletrolyte by LbL. Colloids Surf, 2008; 313:387–392.
LeeS, YeomB, KimY, ChoJ. Layer-by-layer assembly for ultrathin energy-harvesting films: Piezoelectric and triboelectric nanocomposite films. Nano Energy, 2019; 56:1–15.
33.
SyedJA, TangS, MengX. Super-hydrophobic multilayer coatings with layer number tuned swapping in surface wettability and redox catalytic anti-corrosion application. Nature, 2017; 7(4403):1–17.
34.
WaggonerPS, CraigheadHG. The relationship between material properties, device design, and the sensitivity of resonant mechanical sensors. J Appl Phys, 2009; 105:054306.
35.
MargaridoA, MoreiraFMA, HerrmannPSP. Influência da temperatura e umidade no desenvolvimento de um biossensor com microcantilever. Boletim de Pesquisa e Desenvolvimento, 2016; 38(4):1–24.
36.
BrandO, DufourI, HeinrichS, et al.Resonant MEMS: Fundamentals, implementation, and application. Advanced Micro Nanosyst, 2015.
37.
VashistS. A review of microcantilevers for sensing applications. J Nanotechnol, 2007; 3:1–15.
SoneH, OkanoH, HosakaS. Picogram mass sensor using piezoresistive cantilever for biosensor. Jpn J Appl Phys, 2004; 43(7B):4663–4666.
40.
HosakaS, ChiyomaT, IkeuchiA, et. al. Possibility of a femtogram mass biosensor using a self-sensing cantilever. Curr Phys, 2006; 6(3):384–388.
41.
GruberK, HorlacherT, CastelliR, et al.Cantilever array sensors detect specific carbohydrate−protein interactions with picomolar sensitivity. ACS Nano, 2011; 5(5):3670–3678.
42.
MoitraP, BhagatD, PratapR, BhattacharyaS. A novel bio-engineering approach to generate an eminent surface-functionalized template for selective detection of female sex pheromone of Helicoverpa armigera. Sci Rep, 2016; 6(37355):1–12.
