Abstract
Wildberries are recognized worldwide for their nutrition potential, especially for polyphenols such as anthocyanins and flavonoids, which are known as antioxidants. The crowberry, Empetrum nigrum found in northern climates, is a wildberry that has the potential to be used in natural health and cosmetics products due to its high antioxidant activity. This study is focused on antioxidant active crowberries collected from the Basse-Côte-Nord in the province of Québec. The crowberries were analyzed by techniques suitable for a remote region such as the Basse-Côte-Nord, which has limited access to chemicals, equipment and transportation. Two different extraction processes were examined: conventional methods, such as solid-liquid extraction, and unconventional extraction methods such as microwave-assisted extraction. Multiple parameters were tested, including solvent type, solvent-water ratios, liquid/solid ratios, time and temperature. The extracts were analyzed using the Folin-Ciocalteu reagent assay for total phenolic compounds, the pH differential method for total monomeric anthocyanins and the 2,2-diphenyl-1-picrylhydrazyl free radical method for antioxidant activity. The results indicated that, although the ethanol mixtures with water solvent generally gave the highest yield, water extraction gave a significant yield as well. Results also indicated that, compared to the solid-liquid extraction method, the microwave-assisted extraction method allowed for generally smaller liquid:solid ratio (75:1 mL/g compared to 25:1 mL/g with the exception of antioxidant activity) and therefore less solvent. It also allowed for less extraction time (1–5 h compared to 5 min).
Get full access to this article
View all access options for this article.
References
Supplementary Material
Please find the following supplemental material available below.
For Open Access articles published under a Creative Commons License, all supplemental material carries the same license as the article it is associated with.
For non-Open Access articles published, all supplemental material carries a non-exclusive license, and permission requests for re-use of supplemental material or any part of supplemental material shall be sent directly to the copyright owner as specified in the copyright notice associated with the article.
