This work aims to assess the influence of pressurized propane treatment on the enzymatic activity of immobilized inulinases. The effects of system pressure, exposure time, and depressurization rate on the enzymatic activity were evaluated through central composite designs. Propane treatment (3.5 h) increased the residual activity of the non-commercial inulinase from Klyveromyces marxianus NRRL Y-7571 by 176% at 150 bar and a depressurization rate of 60 bar/min. Propane treatment increased the residual activity of Aspergillus niger commercial inulinase by 178% at 270 bar during 6 h exposure at 100 bar/min, the highest depressurization rate. Enzymatic activities changed significantly depending on the enzyme source and the experimental treatment conditions investigated.
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