Xylan is a principal structural component in plant cell walls and could interfere in many industries and biological activities. Biosynthesis of the xylan-degrading enzyme xylanase by fungal isolates was investigated, using agricultural wastes as substrates for production. Aspergillus fumigatus was shown to be the superior isolate to produce xylanase, and wheat bran proved to be the most appropriate substrate for enzyme production and activity. Media composition and growth conditions were optimized for the production of fungal xylanase using Plackett-Burman multifactorial mixture design and Box-Behnken experimental design. Characterization of the purified xylanase revealed that the most favorable conditions for the maximum enzyme activity (57 IU/mL) were temperature of 55-60°C with thermal stability for 30 min and pH of 9 with enzyme stability for 20 min. Application of xylanase for biodegradation of sugarcane pulp fibers was examined using scanning electron micrographs, which exhibited a vigorous alteration in the topography of fiber surface and structure with a prolonged enzymatic treatment period.
JoseleauJP, ComtatJ, RuelK. Chemical structure of xylans and their interactions in the plant cell walls. VisserJ, BeldmanG, vanSomerenMAK, VoragenAGJ. Xylans and Xylanases. Amsterdam, The Netherlands: Elsevier, 1992; 1–15.
5.
PulsJ. Chemistry and biochemistry of hemicelluloses: Relationship between hemicellulose structure and enzymes required for hydrolysis. Macromol Symp, 1997; 120:183–196.
6.
UffenRL. Xylan degradation: A glimpse at microbial diversity. J Ind Microbiol Biotechnol, 1997; 19:1–6.
7.
AhujaSK, FerreiraGM, MoreiraAR. Utilization of enzymes for environmental applications. Crit Rev Biotechnol, 2004; 24,2-3:125–154.
8.
PandeyA, SoccolCR, NigamP, SoccolVT. Biotechnological potential of agro-industrial residues. I, sugar cane bagasse. Bioresour Technol, 2000; 74:69–80.
9.
CleemputG, HessingM, van OortMet al.Purification and characterization of b-D-xylosidase and an endo-xylanase from wheat flour. Plant Physiol, 1997; 113:377–386.
10.
YamuraI, KogaT, MatsumotoT, KatoT. Purification and some properties of endo-1,4-b-D-xylanse from a fresh water mollusc, Pomacea insularus (de Ordigny)Biosci Biotechnol Biochem, 1997; 61:615–620.
11.
BilgramiKS, PandeyAK. Industry and fermentation in introduction to biotechnology. ESK Jain, 1992; 149–165.
12.
WongKKY, TanLUL, SaddlerJN.Multiplicity of β-1,4-xylanase in micro-organisms: Functions and applications. Microbiol Rev, 1998; 52:305–317.
13.
HaqI, KhanA, ButtWAet al.Enhanced production of xylanase by mutant strain of Aspergillus niger GCBMX-45 under solid state fermentation conditions. Indus J Plant Sci, 2002; 1,1:22–27.
14.
Fernández-EspinarM, PiñagaF, de GraaffLet al.Purification, characterization and regulation of the synthesis of an Aspergillus nidulans acidic xylanase. Appl Microbiol Biotechnol, 1994; 42:555–562.
15.
ItoK, IwashitaK, IwanoK. Cloning and sequencing of the xyn C gene encoding acid xylanase of Aspergillus kawachii. Biosci Biotechnol Biochem, 1992; 56:1338–1340.
16.
De SouzaDF, De SouzaCGM, PeraltaRM. Effect of easily metabolizable sugars in the production of xylanase by Aspergillus tamarii in solid-state fermentation. Process Biochem, 2001; 36:835–838.
17.
DuarteMCT, SilvaEC, GomesIMBet al.Purification of Aspergillus sp. xylanase by precipitation with an anionic polymer. Process Biochem, 1999; 34,6,7:577–580.
18.
SavithaS, SadhasivamS, SwaminathanK. Application of Aspergillus fumigatus xylanase for quality improvement of waste paper pulp. Bull Environ Contam Toxicol, 2007; 78:217–221.
19.
GessesseA, MamoG. Purification and characterization of an alkaline xylanase from alkaliphilic Micrococcus sp. AR-135. J Ind Microbiol Biotechnol, 1998; 20:210–214.
20.
WangCH, HanglingY, HaiwanH, HonghaiG. Xylanase production and its application in degradation of hemicellulose materials. Int Cong Biotechnol Pulp Pap Ind, 1998; 7:65–67.
21.
TrinderAB. Micro determination of xylose in plasma. Analyst, 1975; 100:12–15.
22.
PlackettRL, BurmanJP. The design of optimum multifactorial experiments. Biometrica J, 1946; 33:305–325.
23.
MyersRH, MontgomeryDC. Response Surface Methodology: Process and Product Optimization Using Designed Experiments. New York: John Wiley & Sons, 1995.
MohammadiIM. Agricultural waste management extension education (AWMEE.) The ultimate need for intellectual productivity. Am J Environ Sci, 2006; 2,1:10–14.
26.
OkaforUA, OkochiVI, Onyegeme-OkerentaBM, Nwodo-ChineduS. Xylanase production by Aspergillus niger ANL 301 using agrowastes. Afr J Biotechnol, 2007; 6:1710–1714.
27.
DobrevGT, PishtiyskiIG, StanchevVS, MirchevaR. Optimization of nutrient medium containing agricultural wastes for xylanase production by Aspergillus niger B03 using optimal composite experimental design. Bioresour Technol, 2007; 98:2671–2678.
28.
HaltrichD, SteinerW. Formation of xylanase by Schizophyllurn commune: Effect of medium components. Enzyme Microb Technol, 1994; 16:229–235.
29.
ChristovLP, SzakacsG, BalakrishnanH. Production, partial characterization and use of fungal cellulase-free xylanases. Process Biochem, 1999; 34:511–517.
30.
De VriesRP, VisserJ. Aspergillus enzymes involved in degradation of plant cell wall polysaccharides. Microbiol Mol Biol Rev, 2001; 65:497–522.
31.
SinnerM, ParameswaranN, DietrichsHH. Degradation of delignified spruce wood by purified manganese, xylanase, and cellulases. BrownRDJr, JurassekL. Hydrolysis of Cellulose; Mechanism of Enzymatic and Acid Catalysis, Advances in Chemistry Series No. 181. Washington, DC: American Chemical Society, 1979; 303–329.
32.
GrethleinHE. The effect of pore size distribution on the rate of enzymatic hydrolysis of cellulosic substrates. Nat Biotechnol, 1985; 3:155–160.
33.
WongKKY, DeverellKF, MackieKLet al.The relationship between fiber porosity and cellulose digestibility in steam-exploded Pinus radiate. Biotechnol Bioeng, 1988; 31:447–456.
34.
KitpreechavanichV, HayashiM, NagaiS. Purification and properties of endo-1,4-b-xylanase from Humicola lanuginosa. J Ferment Technol, 1984; 5:415–420.
35.
SilvaCHC, PulsJ, SousaMV, Ferreira-FilhoEX. Purification and characterization of a low molecular weight xylanase from solid-state cultures of Aspergillus fumigatus Fresenius. Rev Microbiol, 1999; 30:114–119.
36.
ImmanuelG, BhagavathCMA, IyappaPRet al.Production and partial purification of cellulase by Aspergillus niger and A. fumigatus fermented in coir waste and sawdust. Internet J Micrbiol, 2007; 3,1.
37.
KavitaT, GuptaS, KuhadRC. Properties and application of a partially purified alkaline xylanase from an alkalophilic fungus Aspergillus nidulans KK-99. Bioresour Technol, 2002; 85:39–42.
38.
CarmonaEC, Brochetto-BragaMR, Pizzirani-KleinerAA, JorgeJA. Purification and biochemical characterization of an endoxylanase from Aspergillus versicolor. FEMS Microbiol Lett, 1998; 166:311–315.
39.
UhligH. Industrial Enzymes and Their Applications. New York: John Wiley and Sons, Inc, 1998; 435–461.
40.
DamascoMCT, AndradeCMC, PereiraNJr. Use of corn comb for endoxylanase production by thermophilic fungus Thermomyces lanuginosus IOC-4145. Appl Biochem Biotechnol, 2000; 84-86:821–834.
41.
HuangG. Purification and characterization of an endoxylanase from Trichoderma koningii G-39. Biochem J, 1991; 278:329–333.
42.
GoulartAJ, CarmonaEC, MontiR. Partial purification and properties of cellulose-free alkaline xylanase produced by Rhizopus stolonifer in solid state fermentation. Braz Archives Biol Technol, 2005; 48,3:327–333.
43.
SandrimVC, RizzattiACS, TerenziHFet al.Purification and biochemical characterization of two xylanases produced by Aspergillus caespitosus and their potential for kraft pulp bleaching. Process Biochem, 2005; 40:1823–1828.
44.
SouzaDT, BispoASR, BonEPSet al.Production of thermophilic endo-β-1,4-xylanases by Aspergillus fumigatus FBSPE-05 using agro-industrial by-products. Appl Biochem Biotechnol, 2012; 166:1575–1585.
45.
ChristopherL, BissoonS, SinghSet al.Bleach-enhancing abilities of Thermomyces lanuginosus xylanases produced by solid state fermentation. Process Biochem, 2005; 40:3230–3235.
46.
JiangZQ, LiXT, YangSQet al.Biobleach boosting effect of recombinant xylanase B from the hyperthermophilic Thermotoga maritima on wheat straw pulp. Appl Microbiol Biotechnol, 2006; 70:65–71.
