Abstract
This work is focused on the synthesis of the fructooligosaccharides (FOS) from sucrose using free inulinase from Kluyveromyces marxianus ATCC 16045 in aqueous and aqueous-organic systems. Initially, the influence of five different organic solvents (butyl acetate, ethyl acetate, n-heptane, cyclohexane, and ethanol) on FOS production was investigated. A sequential experimental design methodology was employed to evaluate the effects of temperature, pH, sucrose concentration, inulinase activity, organic solvent content and polyethyleneglycol concentration. Based on the results of the fractional design, two central composite rotational designs were carried out to optimize FOS synthesis in both systems in terms of pH and the sucrose concentration. At optimized conditions, the FOS yield for the aqueous and aqueous-organic systems were 12.7 wt % and 14 wt %, respectively.
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