Abstract
Interleukin-4 (IL-4)– and IL-13–knockout mice were immunized with murine recombinant IL-4 or IL-13, and spleen cells were fused with P3X63-Ag8.653 myeloma cells. Selection of the antigen-positive hybridomas was fulfilled in the presence of IL-6 containing thymic stroma cell supernatant (TSS). All of the selected anti- IL-4– and anti-IL-13–specific hybridoma clones (eight and 10, respectively) required the presence of TSS (0.5–2.5%) for their cloning, stable growth in large-scale cultures, and production of monoclonal antibodies (MAbs). Several of the anti-IL-4–specific clones were adapted to growth without TSS. However, the loss of antibody-secreting capacity in the process of adaptation to TSS-free growth was detected. The data demonstrate that cytokine-deficient mice technology can be used for generation of MAbs to autologous cytokines.
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