Abstract
p50 csk is a protein tyrosine kinase (PTK) that has been reported to regulate the activity of other PTKs belonging to the src gene family. Several hybridoma clones that produce monoclonal antibodies (MAbs) directed against recombinant p50 csk were established. Five of the clones were analyzed for their ability to recognize native and denatured p50 csk protein after undergoing native and denaturing polyacrylamide gel electrophoresis followed by western blotting. In addition, the clones were tested for their ability to immunoprecipitate p50 csk and yet maintain tyrosine kinase activity of antibody-bound p50 csk . None of the clones cross-reacted with pp60 c-src , a PTK that shares with p50 csk the homologous SH1 catalytic domain and SH2 and SH3 regulatory domains. These MAbs can be used to study p50 csk directly, and its role in regulating members of the src family.
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