Immunization of Mice by Injection with a Recombinant Retrovirus Vector Containing Human Factor IX for Production of Monoclonal Antibody Against Factor IX

Abstract

ABSTRACTS

A novel immunization procedure for eliciting murine monoclonal antibodies (mAb) is described. A murine leukemia virus (MLV)-based retroviral vector, designed as a marker protein for the expression of human factor IX(FIX), was constructed. Direct injections of mice with 0.8–1.3 × 10⁴ retroviruses were carried out three times at 4-week intervals. Three out of 4 mice that had the viruses injected subcutaneously produced the antibodies against FIX, and 2 out of 3 mice injected intraperitoneally produced the antibodies. From 1 of the mice with the antibodies, an anti-human FIX murine monoclonal antibody, designated ACTIX (IgM_k type), was produced. The immunization of mice by direct injection of viruses facilitated mAb production in many instances in which cDNAs are available.