Development of Second Generation Monoclonal Antibodies Recognising Lewisy y/b Antigen by Anti-Idiotypic Immunisation

Abstract

Five new monoclonal antibodies (Mabs) recognising the Lewis^y/b hapten of the IgG isotype have been produced following immunisation with rat anti-idiotypic antiserum to C14, an IgM Lewis^y/b hapten Mab and boosting with C14gp200 antigen. They have the same fine specificity as the original Mab binding to a cell surface and secreted antigen preferentially expressed by colorectal tumour cells.