Generation of Murine Triomas Secreting Bi-specific Monoclonal Antibodies That Recognize HBsAG ad and ay Subtypes

Abstract

We report the generation of murine triomas by fusing splenocytes from mice previously immunized with HBsAg αy-subtype and a hybridoma, secreting anti-HBsAg αd-subtype monoclonal antibody, which was rendered HGPRT by induced mutagenesis with N-methyl-N'nitro-N-nitrosoguanidine.

The fusion yielded a 83.8% of hybrids showing the antigen specificity of the parental hybridoma and a 16.1% of bi-specific monoclonal antibodies. One of them, coded as 1C8A5, showing a heavy chain isotype (IgG₁/IgG₂b) was used as capture reagent in an ultramicro-ELISA. As little as O. 78 I. U. of both HBsAg αd- and αy-subtypes could be realiably detected.