Abstract
A mouse monoclonal antibody (MAb 3AE8) of the IgG1 isotype was prepared against rabbit splenocytes and was found by indirect immunofluorescence and direct binding assays to react, in the rabbit, primarily with oil-induced peritoneal exudate macrophages (PEMØ). This MAb did not bind to rabbit T cells, B cells, polymorphonuclear leukocytes, or resident alveolar or peritoneal MØ, but it did bind to a subpopulation of rabbit splenocytes with surface characteristics of null cells. The antibody also recognized mouse and rat PEMØ as well as the murine MØ cell lines P388D1 and IC-21. Consistent with findings in the rabbit, it did not bind to MØ obtained from the peritoneal cavities of normal rats or mice. The addition of MAb 3AE8 to mouse PEMØ caused a marked enhancement in the phagocytic uptake of erythrocyte target cells sensitized with a mouse antierythrocyte antiserum.
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